Investigation of methicillin-resistant Staphylococcus aureus among clinical isolates from humans and animals by culture methods and multiplex PCR

Autor: A. Khair, M. M. Rahman, A. K. M. Anisur Rahman, Naoki Miura, S. M. M. Rahman, M. S. Parvez, Akram Hossain, M. M. Alam, Marzia Rahman, Khaled Bin Amin
Rok vydání: 2018
Předmět:
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
Staphylococcus aureus
040301 veterinary sciences
030106 microbiology
Cattle Diseases
Erythromycin
Microbial Sensitivity Tests
MRSA
Cat Diseases
medicine.disease_cause
Microbiology
0403 veterinary science
03 medical and health sciences
Dogs
Antibiotic resistance
medicine
Animals
Humans
Dog Diseases
Bangladesh
lcsh:Veterinary medicine
General Veterinary
business.industry
Surgical wound
04 agricultural and veterinary sciences
General Medicine
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Ciprofloxacin
Milk
Cats
lcsh:SF600-1100
Cattle
Gentamicin
Coagulase
business
Multiplex Polymerase Chain Reaction
Research Article
medicine.drug
Zdroj: BMC Veterinary Research, Vol 14, Iss 1, Pp 1-6 (2018)
BMC Veterinary Research
ISSN: 1746-6148
DOI: 10.1186/s12917-018-1611-0
Popis: Background Staphylococcus aureus is responsible for large numbers of hospital-related and community-acquired infections. In this study, we investigated the presence of S. aureus and methicillin-resistant S. aureus (MRSA) in 100 samples from animals (55 cattle, 36 dogs, and 9 cats) and 150 samples from hospitalized human patients. The samples were collected from healthy and diseased animals and from diseased humans and included milk, wound swab, pus, exudates, nasal swab and diabetic ulcer. Initially, S. aureus was isolated and identified by colony morphology, Gram staining, and biochemical tests (catalase and coagulase tests). The S. aureus-positive samples were examined by polymerase chain reaction (PCR) to determine their MRSA status. Results Of the 100 animal samples, 29 were positive for S. aureus. Four samples (13.8%) from dogs were MRSA-positive, but samples from cattle and cats were MRSA-negative. Of the 150 human samples we collected, 64 were S. aureus-positive and, of these, 34 (53.1%) were MRSA-positive. Most (28%) of the MRSA samples were isolated from surgical wound swabs, followed by the pus from skin infections (11%), exudates from diabetic ulcers (6%), exudates from burns (4%), and aural swabs (3%). By contrast, a low MRSA detection rate (n = 4) was seen in the non-human isolates, where all MRSA bacteria were isolated from nasal swabs from dogs. The antimicrobials susceptibility testing results showed that S. aureus isolates with mecA genes showed resistance to penicillin (100%), oxacillin (100%), erythromycin (73.5%), ciprofloxacin (70.6%), and gentamicin (67.7%). The lowest resistance was found against ceftazidime, and no vancomycin-resistant isolates were obtained. Conclusions We detected S. aureus and MRSA in both human and canine specimens. Isolates were found to be resistant to some of the antimicrobials available locally. MRSA carriage in humans and animals appears to be a great threat to effective antimicrobials treatment. The prudent use of antimicrobials will reduce the antimicrobial resistance. Our findings will help to find the most appropriate treatment and to reduce antimicrobial resistance in the future by implementing prudent use of antimicrobials. Further studies are required to better understand the epidemiology of MRSA human–animal inter-species transmission in Bangladesh. Electronic supplementary material The online version of this article (10.1186/s12917-018-1611-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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