Genetic mapping identifies loci that influence tomato resistance against Colorado potato beetles
| Autor: | Martha A. Mutschler, John R. Smeda, James J. Giovannoni, Erandi Vargas-Ortiz, Georg Jander, Itay Gonda |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Science Quantitative Trait Loci Quantitative trait locus 01 natural sciences Article 03 medical and health sciences Solanum lycopersicum Glycoalkaloid Gene mapping Animals Inbreeding Leptinotarsa 2. Zero hunger Genetics Multidisciplinary biology fungi Colorado potato beetle Chromosome Mapping food and beverages biology.organism_classification Solanum tuberosum Coleoptera surgical procedures operative 030104 developmental biology Genetic Loci Medicine Solanum Solanaceae 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-24998-5 |
| Popis: | The Colorado potato beetle (CPB; Leptinotarsa decemlineata Say), the most economically important insect pest on potato (Solanum tuberosum L.), also feeds on other Solanaceae, including cultivated tomato (Solanum lycopersicum L.). We used tomato genetic mapping populations to investigate natural variation in CPB resistance. CPB bioassays with 74 tomato lines carrying introgressions of Solanum pennellii in S. lycopersicum cv. M82 identified introgressions from S. pennellii on chromosomes 1 and 6 conferring CPB susceptibility, whereas introgressions on chromosomes 1, 8 and 10 conferred higher resistance. Mapping of CPB resistance using 113 recombinant inbred lines derived from a cross between S. lycopersicum cv UC-204B and Solanum galapagense identified significant quantitative trait loci on chromosomes 6 and 8. In each case, the S. galapagense alleles were associated with lower leaf damage and reduced larval growth. Results of both genetic mapping approaches converged on the same region of chromosome 6, which may have important functions in tomato defense against CPB herbivory. Although genetic mapping identified quantitative trait loci encompassing known genes for tomato acyl sugar and glycoalkaloid biosynthesis, experiments with acyl sugar near-isogenic lines and transgenic GAME9 glycoalkaloid-deficient and overproducing lines showed no significant effect of these otherwise insect-defensive metabolites on CPB performance. |
| Databáze: | OpenAIRE |
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