Association of Mouse Actin-binding Protein 1 (mAbp1/SH3P7), an Src Kinase Target, with Dynamic Regions of the Cortical Actin Cytoskeleton in Response to Rac1 Activation
| Autor: | David G. Drubin, Michael M. Kessels, Åsa E. Y. Engqvist-Goldstein |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Intracellular Fluid
rac1 GTP-Binding Protein Polymers Molecular Sequence Data Arp2/3 complex macromolecular substances Article Cell Line src Homology Domains Mice Actin remodeling of neurons Cell Movement Animals Humans Amino Acid Sequence RNA Messenger Actin-binding protein Cytoskeleton Molecular Biology Platelet-Derived Growth Factor Binding Sites Staining and Labeling biology Microfilament Proteins Cortical actin cytoskeleton Actin remodeling 3T3 Cells Cell Biology Actins Endocytosis Cell biology src-Family Kinases Profilin COS Cells biology.protein Tetradecanoylphorbol Acetate MDia1 Sequence Analysis Cell Division |
| Zdroj: | Molecular Biology of the Cell. 11:393-412 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.11.1.393 |
| Popis: | Yeast Abp1p is a cortical actin cytoskeleton protein implicated in cytoskeletal regulation, endocytosis, and cAMP-signaling. We have identified a gene encoding a mouse homologue of Abp1p, and it is identical to SH3P7, a protein shown recently to be a target of Src tyrosine kinases. Yeast and mouse Abp1p display the same domain structure including an N-terminal actin-depolymerizing factor homology domain and a C-terminal Src homology 3 domain. Using two independent actin-binding domains, mAbp1 binds to actin filaments with a 1:5 saturation stoichiometry. In stationary cells, mAbp1 colocalizes with cortical F-actin in fibroblast protrusions that represent sites of cellular growth. mAbp1 appears at the actin-rich leading edge of migrating cells. Growth factors cause mAbp1 to rapidly accumulate in lamellipodia. This response can be mimicked by expression of dominant-positive Rac1. mAbp1 recruitment appears to be dependent on de novo actin polymerization and occurs specifically at sites enriched for the Arp2/3 complex. mAbp1 is a newly identified cytoskeletal protein in mice and may serve as a signal-responsive link between the dynamic cortical actin cytoskeleton and regions of membrane dynamics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|