Glutathione synthesis in normal liver and in Yoshida AH-130 hepatoma
| Autor: | Maria E. Biocca, Mario U. Dianzani, Giuliana Muzio, Rosa Angela Canuto, Maria Armida Rossi |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
040301 veterinary sciences Glutathione synthesis Toxicology 030226 pharmacology & pharmacy Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liver Neoplasms Experimental Animals Molecular Biology chemistry.chemical_classification Glutathione Disulfide Rats Inbred Strains 04 agricultural and veterinary sciences Cell Biology Glutathione In vitro Glutathione synthetase Liver regeneration Liver Regeneration Rats Enzyme Biochemistry chemistry Liver Rat liver Glutathione disulfide |
| Zdroj: | Toxicologic pathology. 14(4) |
| ISSN: | 0192-6233 |
| Popis: | The amount of reduced glutathione in transplantable hepatomas and in a primary DEN-induced hepatoma is lower than in normal liver. In all tumors examined, the glutathione decrease is not due to an increase of oxidized glutathione. In this paper the in vitro activities of two enzymes involved in glutathione synthesis, γ-glutamylcysteine synthetase and glutathione synthetase, are studied in normal adult rat liver, in regenerating rat liver and in highly anaplastic Yoshida AH-130 hepatoma cells. The activity of these enzymes was determined in the postmicrosomal supernatant fraction as nmoles of [U-14C]-glutamate incorporated into product per mg of soluble protein. In Yoshida AH-130 hepatoma, the γ-glutamylcysteine synthetase and glutathione synthetase activities are lower in respect to normal liver. This is in agreement with the low glutathione content observed in the hepatoma cells. On the other hand, in regenerating liver, there are minimal differences in comparison with normal liver. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|