An Alternative Bioassay for Synchytrium endobioticum Demonstrates the Expression of Potato Wart Resistance in Aboveground Plant Parts
| Autor: | M.P.E. van Gent-Pelzer, Jack H. Vossen, L P van der Gouw, B. T. L. H. van de Vossenberg, Margriet Boerma, T.A.J. van der Lee |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agroinfiltration Synchytrium endobioticum Mycology Plant Science Pathogen proliferation Plant–pathogen interaction 01 natural sciences Biointeractions and Plant Health 03 medical and health sciences Bioassay PBR Resistance in Solanaceae Obligate biology Inoculation Host (biology) fungi food and beverages PBR Breeding for Resistance in Solanaceae Genetics and resistance RNAseq biology.organism_classification Techniques Spore Molecular verification Plant Breeding Horticulture 030104 developmental biology EPS Agronomy and Crop Science Species richness 010606 plant biology & botany |
| Zdroj: | Phytopathology 109 (2019) 6 Phytopathology, 109(6), 1043-1052 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-01-19-0024-r |
| Popis: | The obligate biotrophic chytrid species Synchytrium endobioticum is the causal agent of potato wart disease. Currently, 39 pathotypes have been described based on their interaction with a differential set of potato varieties. Wart resistance and pathotyping is performed using bioassays in which etiolated tuber sprouts are inoculated. Here, we describe an alternative method in which aboveground plant parts are inoculated. Susceptible plants produced typical wart symptoms in developing but not in fully expanded aboveground organs. Colonization of the host by S. endobioticum was verified by screening for resting spores by microscopy and by molecular techniques using TaqMan polymerase chain reaction and RNAseq analysis. When applied to resistant plants, none of these symptoms were detectable. Recognition of S. endobioticum pathotypes by differentially resistant potato varieties was identical in axillary buds and the tuber-based bioassays. This suggests that S. endobioticum resistance genes are expressed in both etiolated “belowground” sprouts and green aboveground organs. RNAseq analysis demonstrated that the symptomatic aboveground materials contain less contaminants compared with resting spores extracted from tuber-based assays. This reduced microbial contamination in the aboveground bioassay could be an important advantage to study this obligate biotrophic plant–pathogen interaction. Because wart resistance is active in both below- and aboveground organs, the aboveground bioassay can potentially speed up screening for S. endobioticum resistance in potato breeding programs because it omits the requirement for tuber formation. In addition, possibilities arise to express S. endobioticum effectors in potato leaves through agroinfiltration, thereby providing additional phenotyping tools for research and breeding. [Formula: see text] Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY 4.0 International license . |
| Databáze: | OpenAIRE |
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