Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer
| Autor: | Andrew Lindsley, Paige Snider, Anthony B. Firulli, Rhonda Rogers, Jian Wang, Brenda Lilly, Simon J. Conway, Hong-Ming Zhou, John B.E. Burch, Shrinagesh V. Koushik, Agnieszka Kruzynska-Frejtag, Michael A. Olaopa |
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| Rok vydání: | 2007 |
| Předmět: |
lacZ reporter mice
Lineage (genetic) Mesenchyme Molecular Sequence Data Schwann cell Mice Transgenic Biology Periostin Mouse embryo Heart development Article Cell Line Mice Fetal Heart Lineage restricted promoter Genes Reporter medicine Animals Amino Acid Sequence Promoter Regions Genetic Enhancer Molecular Biology Conserved Sequence YY1 Transcription Factor Sequence Deletion Binding Sites Base Sequence Sequence Homology Amino Acid Embryogenesis Gene Expression Regulation Developmental Cell Biology Embryonic stem cell Molecular biology Cell biology Mice Inbred C57BL Enhancer Elements Genetic medicine.anatomical_structure Lac Operon Mutagenesis Site-Directed Schwann Cells Peripheral nervous system DNA Probes Endocardial cushions Cell Adhesion Molecules Endocardium Developmental Biology |
| Zdroj: | Developmental Biology. 307(2):340-355 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2007.04.041 |
| Popis: | Periostin is a fasciclin-containing adhesive glycoprotein that facilitates the migration and differentiation of cells that have undergone epithelial–mesenchymal transformation during embryogenesis and in pathological conditions. Despite the importance of post-transformational differentiation as a general developmental mechanism, little is known how periostin's embryonic expression is regulated. To help resolve this deficiency, a 3.9-kb periostin proximal promoter was isolated and shown to drive tissue-specific expression in the neural crest-derived Schwann cell lineage and in a subpopulation of periostin-expressing cells in the cardiac outflow tract endocardial cushions. In order to identify the enhancer and associated DNA binding factor(s) responsible, in vitro promoter dissection was undertaken in a Schwannoma line. Ultimately a 304-bpperi enhancer was identified and shown to be capable of recapitulating 3.9 kbperi-lacZ in vivo spatiotemporal patterns. Further mutational and EMSA analysis helped identify a minimal 37-bp region that is bound by the YY1 transcription factor. The 37-bp enhancer was subsequently shown to be essential for in vivo 3.9 kbperi-lacZ promoter activity. Taken together, these studies identify an evolutionary-conserved YY1-binding 37-bp region within a 304-bp periostin core enhancer that is capable of regulating simultaneous novel tissue-specific periostin expression in the cardiac outflow-tract cushion mesenchyme and Schwann cell lineages. |
| Databáze: | OpenAIRE |
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