Metabolism of (-)-(S)-nicotine in the isolated perfused rabbit lung
| Autor: | C. Marriott, John W. Gorrod, Andrew J. Hutt, A. Bello, G. Aislaitner, S. C. Tan |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Nornicotine Nicotine Chromatography Gas Metabolite Cyanide In Vitro Techniques High-performance liquid chromatography chemistry.chemical_compound medicine Animals Pharmacology (medical) Nicotinic Agonists Lung Biotransformation Chromatography High Pressure Liquid Pharmacology Chromatography Cyanides Stereoisomerism Metabolism chemistry Calibration Rabbits Cotinine Perfusion medicine.drug |
| Zdroj: | European journal of drug metabolism and pharmacokinetics. 22(4) |
| ISSN: | 0378-7966 |
| Popis: | The metabolism of (−)-(S)-nicotine has been investigated following intratracheal administration to the recirculating perfused rabbit lung model. The metabolic products present in the perfusate were identified by co-chromatography (HPLC and GC) with authentic standards and quantified by HPLC. After the 180 min perfusion period, nicotine was found to be metabolically transformed to cotinine (33.7%), 3-hydroxycotinine (10.4%), cotinine-1-N-oxide (3.4%) and nicotine-1′-N-oxide (14.4%). Norcotinine, nornicotine, 3-pyridyl-4-oxo-N-methylbutyramide and an uncharacterised metabolite were also detected in low amounts. Following the perfusion experiment, part of the lung tissue was homogenised in the presence of [14C]-sodium cyanide. Subsequent analysis of the homogenates indicated the formation of 2′-cyanonicotine, 1′-cyanomethylnornicotine and the diastereoisomeric 5′-cyanonicotines. |
| Databáze: | OpenAIRE |
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