Reduced representation sequencing: a success in maize and a promise for other plant genomes
| Autor: | Joseph A. Bedell, W. Brad Barbazuk, Pablo D. Rabinowicz |
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| Rok vydání: | 2005 |
| Předmět: |
Cancer genome sequencing
Comparative genomics Genetics Expressed Sequence Tags DNA Plant Dose-Response Relationship Drug Shotgun sequencing Sequence assembly Genetic Variation Hybrid genome assembly Genomics DNA Sequence Analysis DNA Biology DNA Methylation Genes Plant Zea mays General Biochemistry Genetics and Molecular Biology Deep sequencing Cot analysis Alleles Genome Plant Repetitive Sequences Nucleic Acid |
| Zdroj: | BioEssays : news and reviews in molecular, cellular and developmental biology. 27(8) |
| ISSN: | 0265-9247 |
| Popis: | Summary Plant,andparticularlycerealgenomes,arechallengingto sequence due to their large size and high repetitive DNA content. Gene-enrichment strategies are alternative or complementary approaches to complete genome sequencing that yield, rapidly and inexpensively, useful sequence data from large and complex genomes. The maize genome is large (2.7 Gbp) and contains large amounts of conserved repetitive elements. Furthermore, thehighallelicdiversityfoundbetweenmaizeinbredlines may necessitate sequencing several inbred lines in order to recover the maize ‘‘gene pool’’. Two gene-enrichment approaches, methylation filtration (MF) and high Cot (HC) sequencing have been tested in maize and their ability to sample the gene space has been examined. Combined with other genomic sequencing strategies, gene-enriched genomic sequencing is a practical way to examine the maize gene pool, to order and orient the genic sequences on the genome, and to enable investigation of gene content of other complex plant genomes. BioEssays 27:839–848, 2005. 2005 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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