Xuebijing injection alleviates liver injury by inhibiting secretory function of Kupffer cells in heat stroke rats
| Autor: | Xingqin Zhang, Zhi-guo Pan, Lei Su, Zhi-Feng Liu, Huasheng Tong, Yi Chen, Liqun Tang, Peng-kai Duan |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Heat stroke Liver injury Proinflammatory cytokine Xuebijing Internal medicine Heart rate Animals Humans Medicine Kupffer cells Rats Wistar Medicine(all) Interleukin-6 business.industry Interleukin General Medicine medicine.disease Inflammatory cytokines Rats Systemic inflammatory response syndrome Endocrinology Liver Immunology Hepatic stellate cell Cytokine secretion Tumor necrosis factor alpha business Drugs Chinese Herbal Interleukin-1 |
| Zdroj: | Journal of Traditional Chinese Medicine. 33(2):243-249 |
| ISSN: | 0254-6272 |
| DOI: | 10.1016/s0254-6272(13)60133-8 |
| Popis: | Objective To evaluate the effects of Xuebijing (XBJ) injection in heat stroke (HS) rats and to investigate the mechanisms underlying these effects. Methods Sixty anesthetized rats were randomized into three groups and intravenously injected twice daily for 3 days with 4 mL XBJ (XBJ group) or phosphate buffered saline (HS and Sham groups) per kg body weight. HS was initiated in the HS and XBJ groups by placing rats in a simulated climate chamber (ambient temperature 40°C, humidity 60%). Rectal temperature, aterial pressure, and heart rate were monitored and recorded. Time to HS onset and survival were determined, and serum concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, alanine-aminotransferase (ALT), and aspartate-aminotransferase (AST) were measured. Hepatic tissue was harvested for pathological examination and electron microscopic examination. Kupffer cells (KCs) were separated from liver at HS initiation, and the concentrations of secreted TNF-α, IL-β and IL-6 were measured. Results Time to HS onset and survival were significantly longer in the XBJ than in the HS group. Moreover, the concentrations of TNF-α, IL-1β, IL-6, ALT and AST were lower and liver injury was milder in the XBJ than in the HS group. Heat-stress induced structural changes in KCs and hepatic cells were more severe in the HS than in the XBJ group and the concentrations of TNF-α, IL-β and IL-6 secreted by KCs were lower in the XBJ than in the HS group. Conclusion XBJ can alleviate HS-induced systemic inflammatory response syndrome and liver injury in rats, and improve outcomes. These protective effects may be due to the ability of XBJ to inhibit cytokine secretion by KCs. |
| Databáze: | OpenAIRE |
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