High Labeling Indices of cdc25B is Linked to Progression of Gastric Cancers and Associated With a Poor Prognosis
| Autor: | Koichi Tsuneyama, Eikichi Okada, Yasuo Takano, Hideharu Fujita, Hiroyuki Takahashi, Yoshihiro Murai, Kazuhiro Nomoto |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Histology Lymphovascular invasion Blotting Western Cell Cycle Proteins Cell Fractionation Pathology and Forensic Medicine Stomach Neoplasms Cell Line Tumor Humans cdc25 Phosphatases Medicine Survival analysis Cellular localization Aged business.industry Cancer Prognosis medicine.disease Immunohistochemistry Survival Analysis Blot Medical Laboratory Technology Cytoplasm Multivariate Analysis Disease Progression Cancer research Female Cell fractionation business |
| Zdroj: | Applied Immunohistochemistry & Molecular Morphology. 15:267-272 |
| ISSN: | 1541-2016 |
| Popis: | To clarify the significance of cdc25B, which plays an important physiologic role in regulation of the G2/M check point, in progression of gastric cancer, 125 samples of paraffin-embedded gastric cancers were investigated by immunohistochemistry. In addition, 3 human gastric cancer cell lines were studied to determine the cellular localization of cdc25B by immunohistochemistry and cell fractionation followed by Western blotting. In the cell lines cdc25B was found to be present in both nuclei and cytoplasm, but predominantly in nuclei. High labeling indices of cdc25B in invasion front of gastric cancer was observed in 31 out of 125 cases (24.8%), linked to an advanced depth of cancer invasion (P=0.02), high rates of lymphatic invasion (P=0.03), and lymph node metastasis (P |
| Databáze: | OpenAIRE |
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