Exogenous surfactant treatment before and after sixteen hours of ischemia in experimental lung transplantation
| Autor: | B, Hausen, R, Rohde, C W, Hewitt, F, Schroeder, M, Beuke, R, Ramsamooj, H J, Schäfers, H G, Borst |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Time Factors medicine.medical_treatment Cold storage Myocardial Reperfusion Injury Pulmonary compliance Pulmonary surfactant medicine Animals Lung transplantation Lung Dose-Response Relationship Drug business.industry Airway Resistance Graft Survival Pulmonary Surfactants Rats Transplantation medicine.anatomical_structure Rats Inbred Lew Anesthesia Heart Arrest Induced Vascular resistance Surgery Cardiology and Cardiovascular Medicine business Perfusion Lung Transplantation |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 113:1050-1058 |
| ISSN: | 0022-5223 |
| Popis: | Objective: A syngeneic, acute, double lung transplant model in the rat was used to determine the impact of exogenous surfactant treatment on graft function after prolonged cold storage. Methods: The donor grafts were flush perfused, preserved for 16 hours, and then reperfused for 120 minutes. Untreated lungs served as controls (group I). In group II the recipient received a 200 mg/kg dose of surfactant (CuroSurf) before reperfusion. In groups III and IV, surfactant was administered before perfusion and harvesting (III, 20 mg/kg; IV, 200 mg/kg). Serial measurements of graft pulmonary vascular resistance, alveolar-arterial oxygen difference, and compliance were obtained. Final graft assessment included weight gain and histologic study. Results: Repeated-measures analysis of variance showed significant improvement of graft performance in respect to compliance, alveolar-arterial oxygen difference, and pulmonary vascular resistance in donor surfactant treatment group IV (200 mg/kg) in comparison with recipient treatment (group II) and untreated controls (group I). Reducing the donor surfactant supplementation from 200 mg/kg to 20 mg/kg (group III) improved oxygenation and lung compliance as compared with untreated controls. Grafts in groups I and II had significantly more weight gain after 2 hours of reperfusion. Recipient treatment resulted in significantly more pulmonary hemorrhage in histologic sections. Conclusion: Donor treatment with exogenous surfactant is advantageous for preservation of graft function after extended ischemia. Positive effects may be seen with as little as 20 mg/kg of exogenous surfactant given before donor organ perfusion. (J Thorac Cardiovasc Surg 1997;113:1050-8) |
| Databáze: | OpenAIRE |
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