Additional file 4 of ERAP1 is a critical regulator of inflammasome-mediated proinflammatory and ER stress responses

Autor: Blake, Maja K., O���Connell, Patrick, Pepelyayeva, Yuliya, Godbehere, Sarah, Aldhamen, Yasser A., Amalfitano, Andrea
Rok vydání: 2022
DOI: 10.6084/m9.figshare.19311776.v1
Popis: Additional file 4. Supplemental Figure 4: ASC speck formation analysis with NLRP3 inflammasome stimulation. Bone marrow-derived macrophages (1 �� 106 cells) were plated into 6-well plates, and then cells were primed with LPS (15 ng/ml) for 12 hours. Cells were then stimulated for 30 minutes with Nigericin (Nig.). Cells were stained with Alexa 488-conjugated ASC and DAPI nuclear stain, followed by sample collection and analysis via ImageStream X.
Databáze: OpenAIRE