Cytokine mRNA expression in Peromyscus yucatanicus (Rodentia: Cricetidae) infected by Leishmania (Leishmania) mexicana
| Autor: | Elsy Nalleli Loría-Cervera, Nicole R. Van Wynsberghe, Fernando J. Andrade-Narvaez, Omar A. Saldarriaga, Erika Ivett Sosa-Bibiano, Peter C. Melby |
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| Rok vydání: | 2016 |
| Předmět: |
Peromyscus
Rodent medicine.medical_treatment Leishmania mexicana 030231 tropical medicine Immunology Leishmaniasis Cutaneous Biochemistry Microbiology 03 medical and health sciences 0302 clinical medicine biology.animal medicine Animals Immunology and Allergy Parasite hosting RNA Messenger Molecular Biology Peromyscus yucatanicus Subclinical infection biology Hematology biology.organism_classification Leishmania Cytokine Gene Expression Regulation Cytokines 030215 immunology |
| Zdroj: | Cytokine. 83:176-181 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2016.04.016 |
| Popis: | Peromyscus yucatanicus, the main reservoir of Leishmania (Leishmania) mexicana in the Yucatan peninsula of Mexico, reproduces clinical and histological pictures of LCL in human as well as subclinical infection. Thus, we used this rodent as a novel experimental model. In this work, we analyzed cytokine mRNA expression in P. yucatanicus infected with L. (L.) mexicana. Animals were inoculated with either 2.5×10(6) or 1×10(2) promastigotes and cytokine expressions were analyzed by real-time RT-PCR in skin at 4 and 12weeks post-infection (wpi). Independently of the parasite inoculum none of the infected rodents had clinical signs of LCL at 4wpi and all expressed high IFN-γ mRNA. All P. yucatanicus inoculated with 2.5×10(6) promastigotes developed signs of LCL at 12wpi while the mice inoculated with 1×10(2) remained subclinical. At that time, both IFN-γ and IL-10 were expressed in P. yucatanicus with clinical and subclinical infections. Expressions of TNF-α and IL-4 were significantly higher in clinical animals (2.5×10(6)) compared with subclinical ones (1×10(2)). High TGF-β expression was observed in P. yucatanicus with clinical signs when compared with healthy animals. Results suggested that the clinical course of L. (L.) mexicana infection in P. yucatanicus was associated with a specific local pattern of cytokine production at 12wpi. |
| Databáze: | OpenAIRE |
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