β-Patchoulene, isolated from patchouli oil, suppresses inflammatory mediators in LPS-stimulated RAW264.7 macrophages
| Autor: | Zheng-Quan Lai, Da-Qing Guo, Yu-Hong Liu, Wen-Hao Yang, Xiao-Qi Huang, Jia-Li Liang, Zhi-Xiu Lin, Zi-Ren Su, Qiu-Lin Kong, Yan-Fang Xian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification animal structures Lipopolysaccharide Chemistry Immunology lcsh:R lcsh:Medicine Inflammation Pharmacology Sesquiterpene 03 medical and health sciences chemistry.chemical_compound PATCHOULI OIL 030104 developmental biology 0302 clinical medicine In vivo 030220 oncology & carcinogenesis medicine Immunology and Allergy medicine.symptom Tricyclic |
| Zdroj: | European Journal of Inflammation, Vol 15 (2017) |
| Popis: | β-Patchoulene (β-PAE) is a tricyclic sesquiterpene isolated from patchouli oil. According to our previous study, β-PAE has anti-inflammatory activity in vivo; however, its anti-inflammatory response still remains unconfirmed in vitro. Therefore, this study is committed to demonstrate the anti-inflammatory effect of β-PAE on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. According to our results, pre-treatment with β-PAE significantly decreased the protein and messenger RNA (mRNA) levels of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β while increased the expressions of anti-inflammatory cytokines like IL-10 in a dose-dependent manner. In addition, real-time polymerase chain reaction (PCR) also revealed that β-PAE could interrupt the mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and thus decreased the levels of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 macrophages. In conclusion, these results indicated that β-PAE exerted potent anti-inflammatory activity by maintaining the balance between pro- and anti-inflammatory cytokines as well as suppressing iNOS and COX-2 signaling pathways. |
| Databáze: | OpenAIRE |
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