Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages
| Autor: | Kana Abe, Jing Shu Piao, Riki Toita, Kaori Umezaki, Sayoko Narahara, Masaharu Murata, Kenoki Ohuchida, Lin Cui, Jeong Hun Kang, Makoto Hashizume, Shigekazu Tabata |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Cell Survival Recombinant Fusion Proteins Biophysics Pharmaceutical Science Bioengineering Peptide Nanocapsules Biomaterials Nanocages International Journal of Nanomedicine Drug Discovery Humans drug delivery system Particle Size Protein Precursors hepatocyte cell lines specific Cytotoxicity Heat-Shock Proteins Original Research chemistry.chemical_classification Hepatitis B Surface Antigens Microscopy Confocal Liver cell Organic Chemistry Hep G2 Cells General Medicine Molecular biology In vitro Nanomedicine chemistry Drug delivery Hepatocytes protein nanocages hepatitis B virus HeLa Cells |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Masaharu Murata,1,2 Sayoko Narahara,1,2 Kaori Umezaki,1 Riki Toita,1,2 Shigekazu Tabata,1 Jing Shu Piao,1 Kana Abe,1 Jeong-Hun Kang,3 Kenoki Ohuchida,1,4 Lin Cui,4 Makoto Hashizume1,21Department of Advanced Medical Initiatives, Faculty of Medical Science, Kyushu University, Fukuoka, Japan; 2Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, Japan; 3Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan; 4Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanAbstract: Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.Keywords: protein nanocages, drug delivery system, hepatocyte cell lines specific, hepatitis B virus |
| Databáze: | OpenAIRE |
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