Evaluation of intestinal microbiota, short-chain fatty acids, and immunoglobulin a in diversion colitis
| Autor: | Yosuke Tajima, Kazunao Hayashi, Takeshi Mizusawa, Atsunori Tsuchiya, Yoshifumi Shimada, Ken ichi Mizuno, Hitoshi Kameyama, Kazuya Takahashi, Ayaka Minemura, Kentaro Oka, Shuji Terai, Satoshi Ikarashi, Masato Nakano, Motomichi Takahashi, Kentaro Tominaga, Toshifumi Wakai, Yuzo Kawata, Tomoaki Yosida, Asami Matsumoto, Junji Yokoyama, Hiroki Sato |
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| Rok vydání: | 2021 |
| Předmět: |
AA
acetic acid 0301 basic medicine Immunoglobulin A DC diversion colitis Aerobic bacteria BA butyric acid Biophysics IgA immunoglobulin A SCFAs short-chain fatty acids Biochemistry PA propionic acid Microbiology lcsh:Biochemistry Pathogenesis Short-chain fatty acids 03 medical and health sciences fluids and secretions 0302 clinical medicine Immune system Lactobacillus OA organic acid medicine lcsh:QD415-436 lcsh:QH301-705.5 Diversion colitis biology Microbiota digestive oral and skin physiology IgA - Immunoglobulin A medicine.disease biology.organism_classification digestive system diseases 030104 developmental biology lcsh:Biology (General) 030220 oncology & carcinogenesis biology.protein 16s rrna gene sequencing Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 25, Iss, Pp 100892-(2021) |
| ISSN: | 2405-5808 |
| DOI: | 10.1016/j.bbrep.2020.100892 |
| Popis: | It is reported that an increase in aerobic bacteria, a lack of short-chain fatty acids (SCFAs), and immune disorders in the diverted colon are major causes of diversion colitis. However, the precise pathogenesis of this condition remains unclear. The aim of the present study was to examine the microbiota, intestinal SCFAs, and immunoglobulin A (IgA) in the diverted colon. Eight patients underwent operative procedures for colostomies. We assessed the diverted colon using endoscopy and obtained intestinal samples from the diverted colon and oral colon in these patients. We analyzed the microbiota and SCFAs of the intestinal samples. The bacterial communities were investigated using a 16S rRNA gene sequencing method. The microbiota demonstrated a change in the proportion of some species, especially Lactobacillus, which significantly decreased in the diverted colon at the genus level. We also showed that intestinal SCFA values were significantly decreased in the diverted colon. Furthermore, intestinal IgA levels were significantly increased in the diverted colon. This study was the first to show that intestinal SCFAs were significantly decreased and intestinal IgA was significantly increased in the diverted colon. Our data suggest that SCFAs affect the microbiota and may play an immunological role in diversion colitis. Highlights • Anaerobic bacteria like Lactobacillus significantly decreased in the diverted colon. • Fecal short-chain fatty acids significantly decreased in the diverted colon. • Acetic acid also decreased significantly in the diverted colon. • Fecal immunoglobulin A levels were significantly increased in the diverted colon. |
| Databáze: | OpenAIRE |
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