Directed migration of human bone marrow mesenchymal stem cells in a physiological direct current electric field
| Autor: | Colin D. McCaig, Iain R. Gibson, Carolyn Watt, Alexandra Karystinou, Cosimo De Bari, Zhiqiang Zhao, Anke J. Roelofs |
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| Rok vydání: | 2011 |
| Předmět: |
Senescence
Bone Regeneration Cell Survival Cell Bone Marrow Cells Bone healing Biology Time-Lapse Imaging Young Adult Cell Movement Osteogenesis medicine Humans Bone regeneration Cells Cultured Cellular Senescence Microscopy Video Mesenchymal stem cell Cell migration Mesenchymal Stem Cells Bone fracture Middle Aged medicine.disease In vitro Electric Stimulation Cell biology medicine.anatomical_structure Phenotype Biomedical engineering |
| Zdroj: | European cellsmaterials. 22 |
| ISSN: | 1473-2262 |
| Popis: | At sites of bone fracture, naturally-occurring electric fields (EFs) exist during healing and may guide cell migration. In this study, we investigated whether EFs could direct the migration of bone marrow mesenchymal stem cells (BM-MSCs), which are known to be key players in bone formation. Human BM-MSCs were cultured in direct current EFs of 10 to 600 mV/mm. Using time-lapse microscopy, we demonstrated that an EF directed migration of BM-MSCs mainly to the anode. Directional migration occurred at a low threshold and with a physiological EF of ~25 mV/mm. Increasing the EF enhanced the MSC migratory response. The migration speed peaked at 300 mV/mm, at a rate of 42 ±1 µm/h, around double the control (no EF) migration rate. MSCs showed sustained response to prolonged EF application in vitro up to at least 8 h. The electrotaxis of MSCs with either early (P3-P5) or late (P7-P10) passage was also investigated. Migration was passage-dependent with higher passage number showing reduced directed migration, within the range of passages examined. An EF of 200 mV/mm for 2 h did not affect cell senescence, phenotype, or osteogenic potential of MSCs, regardless of passage number within the range tested (P3-P10). Our findings indicate that EFs are a powerful cue in directing migration of human MSCs in vitro. An applied EF may be useful to control or enhance migration of MSCs during bone healing. |
| Databáze: | OpenAIRE |
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