Synthesis and in Vitro Degradation of New Polyvalent Hydrazide Cross-Linked Hydrogels of Hyaluronic Acid
| Autor: | Glenn D. Prestwich, James F. Marecek, Koen Vercruysse, Dale M. Marecak |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Magnetic Resonance Spectroscopy
Chemical Phenomena Molecular Sequence Data Biomedical Engineering Hyaluronoglucosaminidase Pharmaceutical Science Bioengineering Hydrazide complex mixtures Chemical synthesis Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols chemistry.chemical_compound Hyaluronidase Polymer chemistry Hyaluronic acid medicine Hyaluronic Acid Biotransformation Carbodiimide Pharmacology biology Chemistry Physical Chemistry Organic Chemistry technology industry and agriculture Hydrogen-Ion Concentration biology.organism_classification Cross-Linking Reagents Carbohydrate Sequence Cross linked hydrogels Self-healing hydrogels Tetra Gels Biotechnology medicine.drug |
| Zdroj: | Bioconjugate Chemistry. 8:686-694 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | New polyvalent hydrazide cross-linkers were synthesized, characterized, and used to prepare hydrazide cross-linked hydrogels derived from hyaluronic acid (HA). First, the chemical synthesis and characterization of the di-, tri-, tetra-, penta-, and hexahydrazides are presented. Second, HA concentration, buffer type and concentration, and ratio of HA to carbodiimide to cross-linker were varied to obtain HA-hydrogels with different chemical and physical properties. Third, two new assays are described to monitor the stability of HA-hydrogels toward hyaluronidase (HAse) and other media. These assays were used to evaluate the stability of cross-linked HA-hydrogels to HAse solutions and different pH values. Hydrophobic cross-linkers gave the most stable gels, and the susceptibility of the gels to HAse was independent of cross-linker concentration. HAse does not significantly penetrate the HA-hydrogels and acts primarily at the gel-solution interface. The HA-hydrogels are stable in acid environments and dissolve gradually above pH 7.0. |
| Databáze: | OpenAIRE |
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