Construction and characterisation of infectious recombinant HIV-1 clones containing CTL epitopes from structural proteins in Nef
| Autor: | Carel A. van Baalen, Esther J. Verschuren, Christophe Guillon, Albert D. M. E. Osterhaus, Patrick H. M. Boers, Rob A. Gruters |
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| Přispěvatelé: | Virology |
| Rok vydání: | 2001 |
| Předmět: |
Viral protein
viruses Epitopes T-Lymphocyte Biology medicine.disease_cause Virus Replication Virus Epitope Gene Products nef law.invention Cell Line SDG 3 - Good Health and Well-being law Virology medicine Humans Amino Acid Sequence nef Gene Products Human Immunodeficiency Virus Cloning Molecular Peptide sequence Recombination Genetic virus diseases Gene Products env Reverse transcriptase HIV Reverse Transcriptase CTL Viral replication Recombinant DNA HIV-1 T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Virological Methods, 99, 115-121. Elsevier |
| ISSN: | 0166-0934 |
| Popis: | In this study the construction is described of HIV-1 molecular clones in which CTL epitopes from RT or Env late proteins were inserted into the Nef early protein. The ectopic epitopes were efficiently processed from the recombinant Nef proteins, were recognized by their cognate CTL in cytolytic assays, and did not perturb virus replication or viral protein expression in vitro. These recombinant viruses will therefore be an important tool in studying the effect of distinct epitope expression kinetics on the efficiency of CTL-mediated suppression of HIV-1 replication. |
| Databáze: | OpenAIRE |
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