DNA damage caused by etoposide and γ-irradiation induces gene conversion of the MHC in a mouse non-germline testis cell line
| Autor: | Kari Högstrand, Jan Böhme |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
DNA repair DNA damage Health Toxicology and Mutagenesis DNA Mutational Analysis Genes MHC Class II Molecular Sequence Data Gene Conversion Endogeny Biology Mice chemistry.chemical_compound Testis Genetics medicine Animals Amino Acid Sequence Gene conversion Molecular Biology Gene Cells Cultured Crosses Genetic Etoposide Mice Inbred BALB C Mice Inbred C3H Base Sequence Dose-Response Relationship Drug Dose-Response Relationship Radiation Molecular biology chemistry Gamma Rays Cell culture Sequence Alignment DNA DNA Damage medicine.drug |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 423:155-169 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/s0027-5107(98)00239-5 |
| Popis: | We have explored the effects of gamma-irradiation and etoposide on the gene conversion frequency between the endogenous major histocompatibility complex class II genes Abk and Ebd in a mouse testis cell line of non-germline origin with a polymerase chain reaction assay. Both gamma-rays and etoposide were shown to increase the gene conversion frequency with up to 15-fold compared to untreated cells. Etoposide, which is an agent that stabilise a cleavable complex between DNA and DNA topoisomerase II, shows an increased induction of gene conversion events with increased dose of etoposide. Cells treated with gamma-rays, which induce strand breaks, had an increased gene conversion frequency when they were subjected to low doses of irradiation, but increasing doses of irradiation did not lead to an increase of gene conversion events, which might reflect differences in the repair process depending on the extent and nature of the DNA damage. These results where DNA damage was shown to be able to induce gene conversion of endogenous genes in mouse testis cells suggests that the DNA repair system could be involved in the molecular genetic mechanism that results in gene conversion in higher eukaryotes like mammals. |
| Databáze: | OpenAIRE |
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