Host genetic background effect on the frequency of mouse mammary tumor virus-induced rearrangements of the int-1 and int-2 loci in mouse mammary tumors
| Autor: | Gill Campbell, F Squartini, James M. Robbins, M. Bistocchi, Robert Callahan, F Buttitta, A Marchetti |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Ratón viruses Immunology Mammary gland Molecular Sequence Data Microbiology Virus Insertional mutagenesis Mice Virology medicine Animals Inbreeding RNA Messenger Repetitive Sequences Nucleic Acid Gene Rearrangement Mice Inbred BALB C Mice Inbred C3H biology Base Sequence Mouse mammary tumor virus Mammary Neoplasms Experimental Gene rearrangement Provirus biology.organism_classification Blotting Northern Molecular biology Blotting Southern Mutagenesis Insertional medicine.anatomical_structure Mammary Epithelium Mammary Tumor Virus Mouse Insect Science DNA Viral RNA Female DNA Probes Poly A Research Article |
| Popis: | The frequency with which int-1 and int-2 are rearranged in mouse mammary tumors by mouse mammary tumor virus (MMTV)-induced insertional mutagenesis is a consequence of the host genetic background. In 75% of C3H mammary tumors, int-1 is rearranged by MMTV insertion, whereas only 30% of BALB/cfC3H tumors contain a virus-induced rearrangement of int-1. This difference is significant (P less than 0.005) and could not be accounted for by the potentially additive effect of the genetically transmitted Mtv-1-encoded virus in C3H mice. Similarly, MMTV-induced rearrangement of the int-2 gene in mammary tumors of the R111 mouse strain (59%) occurred at a significantly (P less than 0.025) higher frequency than in BALB/cfR111 (25%) mammary tumors. Moreover, in BALB/cfR111 mammary tumors, there is evidence that rearrangement of int-1 and int-2 does not occur independently (P less than 0.025). These results suggest that the long history of inbreeding for high tumor incidence of C3H and R111 mouse strains has selected for the fixation of host mutations which either complement the action of the particular int gene or affect the sensitivity of specific subpopulations of mammary epithelium to infection by particular strains of MMTV. |
| Databáze: | OpenAIRE |
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