Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery
| Autor: | Nikica Zaninovic, Michael Ginsberg, R. Bodine, Laura Park, Daylon James, Glenn L. Schattman, Limor Man, Robert E. Schwartz, Zev Rosenwaks |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system General Chemical Engineering Bioengineering Context (language use) Ovary General Biochemistry Genetics and Molecular Biology Cryopreservation Mice 03 medical and health sciences Paracrine signalling 0302 clinical medicine Tissue engineering Paracrine Communication Animals Humans Medicine Ovarian tissue cryopreservation Fertility preservation 030219 obstetrics & reproductive medicine Tissue Engineering General Immunology and Microbiology business.industry General Neuroscience Endothelial Cells Antral follicle 030104 developmental biology medicine.anatomical_structure Cancer research Female business |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| DOI: | 10.3791/57472 |
| Popis: | Infertility is a frequent side effect of chemotherapy and/or radiotherapy and for some patients, cryopreservation of oocytes or embryos is not an option. As an alternative, an increasing number of these patients are choosing to cryopreserve ovarian tissue for autograft following recovery and remission. Despite improvements in outcomes among patients undergoing auto-transplantation of cryopreserved ovarian tissue, efficient revascularization of grafted tissue remains a major obstacle. To mitigate ischemia and thus improve outcomes in patients undergoing auto-transplantation, we developed a vascular cell-based strategy for accelerating perfusion of ovarian tissue. We describe a method for co-transplantation of exogenous endothelial cells (ExECs) with cryopreserved ovarian tissue in a mouse xenograft model. We extend this approach to employ ExECs that have been engineered to constitutively express Anti-Mullerian hormone (AMH), thus enabling sustained paracrine signaling input to ovarian grafts. Co-transplantation with ExECs increased follicular volume and improved antral follicle development, and AMH-expressing ExECs promoted retention of quiescent primordial follicles. This combined strategy may be a useful tool for mitigating ischemia and modulating follicular activation in the context of fertility preservation and/or infertility at large. |
| Databáze: | OpenAIRE |
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