Overexpression of Arabidopsis ACBP3 enhances NPR1-dependent plant resistance to Pseudomonas syringe pv tomato DC3000
| Autor: | Mee-Len Chye, Shi Xiao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Plant Diseases - Immunology - Microbiology
Physiology Mutant Arabidopsis Intracellular Space Salicylic Acid - Metabolism Pseudomonas syringae Plant Science Gene Knockout Techniques Green Fluorescent Proteins - Metabolism Plant Growth Regulators Gene Expression Regulation Plant Plant defense against herbivory Arabidopsis thaliana Intracellular Space - Drug Effects - Metabolism Botrytis cinerea Disease Resistance Oligonucleotide Array Sequence Analysis Arachidonic Acid biology Cell Death Pseudomonas Pseudomonas Syringae - Drug Effects - Physiology Hydrogen Peroxide - Metabolism Plant Growth Regulators - Pharmacology Gene Expression Regulation Plant - Drug Effects Phenotype Arachidonic Acid - Pharmacology Botrytis Cell Death - Drug Effects Salicylic Acid Green Fluorescent Proteins Arabidopsis - Drug Effects - Genetics - Immunology - Microbiology Genes Plant Arabidopsis Proteins - Genetics - Metabolism Microbiology Genetics Plants Interacting with Other Organisms RNA Messenger Plant Diseases Arabidopsis Proteins fungi Wild type Hydrogen Peroxide Genes Plant - Genetics biology.organism_classification Disease Resistance - Drug Effects - Immunology Botrytis - Drug Effects - Physiology Rna Messenger - Genetics - Metabolism Carrier Proteins - Genetics - Metabolism Carrier Proteins |
| Zdroj: | Plant physiology. 156(4) |
| ISSN: | 1532-2548 |
| Popis: | ACBP3 is one of six Arabidopsis (Arabidopsis thaliana) genes, designated ACBP1 to ACBP6, that encode acyl-coenzyme A (CoA)-binding proteins (ACBPs). These ACBPs bind long-chain acyl-CoA esters and phospholipids and are involved in diverse cellular functions, including acyl-CoA homeostasis, development, and stress tolerance. Recombinant ACBP3 binds polyunsaturated acyl-CoA esters and phospholipids in vitro. Here, we show that ACBP3 plays a role in the plant defense response to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. ACBP3 mRNA was up-regulated upon pathogen infection and treatments using pathogen elicitors and defense-related phytohormones. Transgenic Arabidopsis ACBP3 overexpressors (ACBP3-OEs) showed constitutive expression of pathogenesis-related genes (PR1, PR2, and PR5), cell death, and hydrogen peroxide accumulation in leaves. Consequently, ACBP3-OEs displayed enhanced resistance to the bacterial pathogen P. syringae DC3000. In contrast, the acbp3 T-DNA insertional mutant was more susceptible and exhibited lower PR gene transcript levels upon infection. Using the ACBP3 OE-1 line in combination with nonexpressor of PR genes1 (npr1-5) or coronatine-insensitive1 (coi1-2), we concluded that the enhanced PR gene expression and P. syringae DC3000 resistance in the ACBP3-OEs are dependent on the NPR1-mediated, but not the COI1-mediated, signaling pathway. Given that ACBP3-OEs showed greater susceptibility to infection by the necrotrophic fungus Botrytis cinerea while the acbp3 mutant was less susceptible, we suggest that ACBP3 plays a role in the plant defense response against biotrophic pathogens that is distinct from necrotrophic pathogens. ACBP3 function in plant defense was supported further by bioinformatics data showing up-regulation of many biotic and abiotic stress-related genes in ACBP3 OE-1 in comparison with the wild type. © 2011 American Society of Plant Biologists. link_to_OA_fulltext |
| Databáze: | OpenAIRE |
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