Algoriphagus aquaeductus sp. nov., isolated from a freshwater pipe
| Autor: | Jan Erik Rau, Ulrich Fischer, Karl-Heinz Blotevogel |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Stereochemistry Sequence analysis Molecular Sequence Data Sodium Chloride DNA Ribosomal Microbiology RNA Ribosomal 16S Botany Benzoquinones Cluster Analysis Microaerophile Bacterial Capsules Phospholipids Phylogeny Ecology Evolution Behavior and Systematics chemistry.chemical_classification Base Composition Oxidase test biology Strain (chemistry) Bacteroidetes Drinking Water Fatty Acids Temperature Nucleic Acid Hybridization Pigments Biological Sequence Analysis DNA General Medicine Hydrogen-Ion Concentration Ribosomal RNA Catalase biology.organism_classification Carotenoids Aerobiosis Carbon Bacterial Typing Techniques Amino acid chemistry Algoriphagus Energy Metabolism Oxidoreductases Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 62:675-682 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.030809-0 |
| Popis: | A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4T, was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15–42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4T tolerated concentrations of 0–4 % (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C15 : 0 (32.2 %), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 22.5 %), and iso-C17 : 0 3-OH (7.9 %). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA G+C content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4T to the CFB group, forming a coherent cluster with species of the genus Algoriphagus with the highest similarity of 98.8 % to Algoriphagus aquatilis A8-7T. DNA–DNA hybridization revealed 37.5 % relatedness to strain A8-7T. Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4T should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus aquaeductus sp. nov. (type strain T4T = DSM 19759T = LMG 24398T = NCIMB 14399T) is proposed. |
| Databáze: | OpenAIRE |
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