Ursodeoxycholate Restores Biliary Excretion of Methotrexate in Rats with Ethinyl Estradiol Induced-Cholestasis by Restoring Canalicular Mrp2 Expression
| Autor: | Yun Ju Kang, Hye-Young Chi, Im-Sook Song, Min-Koo Choi, Hye Hyun Yoo, Min Ju Kim, Mihwa Kwon, Chang-Koo Shim, Young A. Choi |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Administration Oral Pharmacology Ethinyl Estradiol ursodeoxycholate (UDCA) intrahepatic cholestasis multidrug resistance-associated protein (Mrp) 2 methotrexate (MTX) biliary excretion clearance lcsh:Chemistry Bile flow 0302 clinical medicine lcsh:QH301-705.5 Spectroscopy Cholestasis Multidrug resistance-associated protein 2 Ursodeoxycholic Acid Ursodeoxycholate General Medicine Multidrug Resistance-Associated Protein 2 Computer Science Applications Treatment Outcome 030211 gastroenterology & hepatology medicine.drug Down-Regulation Hepatobiliary Elimination Drug Administration Schedule Article Catalysis Bile Acids and Salts Inorganic Chemistry 03 medical and health sciences Biliary excretion In vivo medicine Animals Physical and Theoretical Chemistry Molecular Biology business.industry Organic Chemistry medicine.disease Rats Methotrexate 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 ATP-Binding Cassette Transporters business |
| Zdroj: | International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 1120 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 4, p 1120 (2018) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19041120 |
| Popis: | The in vivo relevance of ursodeoxycholate (UDCA) treatment (100 mg/kg/day, per oral tid for 5 days before cholestasis induction followed by the same dosing for 5 days) on hepatic function was investigated in rats with 17α-ethinylestradiol (EE, 10 mg/kg, subcutaneous for 5 days)-induced experimental cholestasis. The bile flow rate and the expression level of hepatic multidrug resistance-associated protein 2 (Mrp 2) that were decreased in cholestasis were restored after UDCA treatment. Consistent with this, the biliary excretion clearance (CLexc,bile) of a representative Mrp2 substrate—methotrexate (MTX)—was decreased in cholestatic rats but was restored after UDCA treatment. Consequently, the plasma concentrations of MTX, which were increased by cholestasis, were decreased to control levels by UDCA treatment. Thus, the restoration of CLexc,bile appears to be associated with the increase in Mrp2 expression on the canalicular membrane by UDCA treatment followed by Mrp2-mediated biliary excretion of MTX. On the other hand, the hepatic uptake clearance (CLup,liver) of MTX was unchanged by cholestasis or UDCA treatment, suggestive of the absence of any association between the uptake process and the overall biliary excretion of MTX. Since UDCA has been known to induce the expression of canalicular MRP2 in humans, UDCA treatment might be effective in humans to maintain or accelerate the hepatobiliary elimination of xenobiotics or metabolic conjugates that are MRP2 substrates. |
| Databáze: | OpenAIRE |
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