Detection of SARS-CoV-2 RNA in bivalve mollusks and marine sediments
| Autor: | Jesús L. Romalde, María Teresa Fernández-Núñez, Marta Lois, David Polo |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Universidade de Santiago de Compostela. Instituto Interdisciplinar de Tecnoloxías Ambientais (CRETUS) |
| Rok vydání: | 2021 |
| Předmět: |
Geologic Sediments
Environmental Engineering 010504 meteorology & atmospheric sciences Zoology Ruditapes 010501 environmental sciences Wastewater 01 natural sciences Article Virus Bacteriophage Sediments Animals Humans Environmental Chemistry Waste Management and Disposal Marine environment 0105 earth and related environmental sciences biology SARS-CoV-2 Sediment RNA COVID-19 Contamination crAssphage Bivalvia biology.organism_classification Pollution Bivalve mollusks RNA Viral |
| Zdroj: | Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname The Science of the Total Environment |
| Popis: | The presence of SARS-CoV-2 in wastewater pose the question of whether this new pandemic virus could be released into watercourses and potentially continue to finally reach coastal waters. In this study, we employed two bivalve molluscan species from the genus Ruditapes as sentinel organisms to investigate the presence of SARS-CoV-2 signals in the marine coastal environment. Estuarine sediments from the natural clam banks were also analyzed. Viral RNA was detected by RT-qPCR, targeting IP4, E and N1 genomic regions. Positive samples were also subjected to a PMAxx-triton viability RT-qPCR assay in order to discriminate between intact and altered capsids, obtaining indirect information about the viability of the virus. SARS-CoV-2 RNA traces were detected in 9/12 clam samples by RT-qPCR, from which 4 were positive for two different target regions. Viral quantification ranged from Graphical abstract Unlabelled Image |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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