Evaluation of the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) as a Screening Method for the Detection of Influenza Viruses in the Fecal Materials of Water Birds
| Autor: | Masayuki Motoshima, Mayumi Endo, Fumi Yoshino, Yoshihiro Sakoda, Hiromi Yoshida, Masatoshi Okamatsu, Hiroshi Kida, Takahiro Soejima, Naoki Yamamoto, Syouhei Senba, Hidetoshi Kanda |
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| Rok vydání: | 2011 |
| Předmět: |
Feces/virology
Influenza A Virus H3N2 Subtype/isolation & purification Viral Matrix Proteins/genetics viruses Loop-mediated isothermal amplification Sensitivity and Specificity Biology medicine.disease_cause Nucleic Acid Amplification Techniques/veterinary Microbiology Viral Matrix Proteins Feces medicine Animals Natural reservoir Gene Reverse Transcription Loop-mediated Isothermal Amplification Phylogeny RNA Viral/genetics Disease Reservoirs Viral Matrix Proteins/chemistry Reverse Transcription Influenza in Birds/diagnosis General Veterinary Influenza A Virus H3N2 Subtype Influenza in Birds/virology Disease Reservoirs/veterinary RNA Nucleic Acid Amplification Techniques/methods Virology Influenza A virus subtype H5N1 Reverse transcriptase RNA Viral/chemistry Ducks Influenza in Birds RNA Viral Nucleic Acid Amplification Techniques Influenza A Virus H3N2 Subtype/genetics |
| Zdroj: | Journal of Veterinary Medical Science. 73:753-758 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.10-0505 |
| Popis: | Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day. |
| Databáze: | OpenAIRE |
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