Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters
| Autor: | Fernanda Gatti de Oliveira Nascimento, Marlus Alves dos Santos, Guilherme M.J. Costa, Bruna Cristina Borges, Antonio Vicente Mundim, Ana Flávia Oliveira Notário, Rebecca Tavares e. Silva, Maria Aparecida de Souza, Claudio Vieira da Silva, Andre Belico Vasconcelos, Amanda Pifano Neto Quintal, Paula Cristina Brígido |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine endocrine system 040301 veterinary sciences Short Report Genitalia Male urologic and male genital diseases 0403 veterinary science 03 medical and health sciences Immune system Cricetinae Testis parasitic diseases Animals Humans Medicine Testosterone Sex organ Immune response Leishmania Epididymis Azoospermia Mesocricetus Testicular atrophy biology Leishmania chagasi business.industry Public Health Environmental and Occupational Health Leishmaniasis 04 agricultural and veterinary sciences General Medicine Extracellular amastigotes medicine.disease biology.organism_classification Disease Models Animal Kinetics 030104 developmental biology Infectious Diseases medicine.anatomical_structure Immunology Cytokines Leishmaniasis Visceral business |
| Zdroj: | Infectious Diseases of Poverty |
| ISSN: | 2049-9957 |
| DOI: | 10.1186/s40249-016-0122-0 |
| Popis: | Background Leishmaniasis causes alterations and lesions in the genital system, which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection. The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters (Mesocricetus auratus). Methods Animals were intraperitoneally inoculated with amastigotes from L. chagasi. At different time points animals were euthanized and genital organs processed for histo-pathological, qPCR, cytokines and testosterone detection assays. Results Our results showed a high parasite load in testis, followed by an increase of pro-inflammatory cytokines IL1-β, TNF-α and IFN-γ, and testosterone. Subsequently, IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach. Conclusion Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L. chagasi infection in hamsters. Electronic supplementary material The online version of this article (doi:10.1186/s40249-016-0122-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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