Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA
| Autor: | Y Hayakawa, Kenichi Matsubara, Tomoaki Murotsu |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
DNA Replication
DNA Bacterial Biology Microbiology DNA-binding protein F Factor chemistry.chemical_compound Plasmid Bacterial Proteins Genes Regulator Escherichia coli Binding site Molecular Biology Gene F-factor Binding Sites DNA replication Molecular biology PBR322 DNA-Binding Proteins Molecular Weight Gene Expression Regulation Genes chemistry Genes Bacterial Chromosome Deletion DNA Research Article |
| Zdroj: | Journal of Bacteriology. 163:349-354 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.163.1.349-354.1985 |
| Popis: | A mini-F-coded protein, named F2 protein, binds specifically to mini-F DNA. This protein has a molecular weight of 37,000 and is coded by the A2 segment of the mini-F genome (47.3 to 49.4 kilobases on the F coordinate map). The binding site is located also in the A2 segment of mini-F. This binding site is lost by spontaneous deletion when the A2 segment alone, but not A2 together with its neighboring segment, is cloned in a multicopy plasmid pBR322. These data are discussed in connection with incompatibility and plasmid stability. |
| Databáze: | OpenAIRE |
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