Metabolic Reprogramming of Trophoblast Cells in Response to Hypoxia

Autor: I M Tsypina, E N Knyazev, Gennady T. Sukhikh, Galina S. Zakharova, L. A. Astakhova, Alexander G. Tonevitsky
Rok vydání: 2019
Předmět:
0301 basic medicine
Nitric Oxide Synthase Type III
Cell
Protein Serine-Threonine Kinases
Mitochondrion
Models
Biological
Oxidative Phosphorylation
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Pre-Eclampsia
Pregnancy
Cell Line
Tumor
Proto-Oncogene Proteins
Placenta
medicine
Humans
Chemistry
Gene Expression Profiling
Choriocarcinoma
Membrane Proteins
Pyruvate Dehydrogenase Acetyl-Transferring Kinase
RNA-Binding Proteins
Trophoblast
General Medicine
Hypoxia (medical)
Cellular Reprogramming
Oxyquinoline
medicine.disease
Cell Hypoxia
Trophoblasts
Cell biology
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
embryonic structures
Female
medicine.symptom
Carrier Proteins
Glycolysis
Reprogramming
030217 neurology & neurosurgery
Zdroj: Bulletin of Experimental Biology and Medicine. 166:321-325
ISSN: 1573-8221
0007-4888
Popis: Hypoxia of trophoblast cells is an important regulator of normal development of the placenta. However, some pathological states associated with hypoxia, e.g. preeclampsia, impair the functions of placental cells. Oxyquinoline derivative inhibits HIF-prolyl hydroxylase by stabilizing HIF-1 transcription complex, thus modeling cell response to hypoxia. In human choriocarcinoma cells BeWo b30 (trophoblast model), oxyquinoline increased the expression of a core hypoxia response genes along with up-regulation of NOS3, PDK1, and BNIP3 genes and down-regulation of the PPARGC1B gene. These changes in the expression profile attest to activation of the metabolic cell reprogramming mechanisms aimed at reducing oxygen consumption by enabling the switch from aerobic to anaerobic glucose metabolism and the respective decrease in number of mitochondria. The possibility of practical use of the therapeutic properties of oxyquinoline derivatives is discussed.
Databáze: OpenAIRE
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