Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment
| Autor: | Uwe Irion, Anastasia Eskova, Joan Cerdà, Christiane Nüsslein-Volhard, Hans-Martin Maischein, François Chauvigné, Moritz Ammelburg |
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| Přispěvatelé: | Producció Animal, Aqüicultura, Max Planck Society |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mutant Green Fluorescent Proteins Mutation Missense Aquaporin Biology Aquaporins Permeability 03 medical and health sciences Pigment 0302 clinical medicine Missense mutation Animals Amino Acid Sequence Chromatophores RNA Messenger Allele Molecular Biology Zebrafish Genes Dominant Genetics Aquaporin 3 Pigmentation Neural crest Zebrafish Proteins biology.organism_classification Phenotype Cell biology 030104 developmental biology visual_art Mutation visual_art.visual_art_medium Animal Fins Pattern formation 030217 neurology & neurosurgery Developmental Biology Research Article |
| Zdroj: | Development (Cambridge, England) IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 11 pages, 6 figures, 1 table, supplementary information https://dx.doi.org/10.1242/dev.143495 The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. Many of the known mutations that alter the pattern act cell-autonomously in pigment cells, and our knowledge about external regulators is limited. Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that lead to broken stripes and short fins. A loss-of-function aqp3a allele, generated by CRISPR-Cas9, has no phenotypic consequences, demonstrating that Aqp3a is dispensable for normal development. Strikingly, the pigment cells from dominant mau mutants are capable of forming a wild-type pattern when developing in a wild-type environment, but the surrounding tissues in the mutants influence pigment cell behaviour and interfere with the patterning process. The mutated amino acid residues in the dominant alleles line the pore surface of Aqp3a and influence pore permeability. These results demonstrate an important effect of the tissue environment on pigment cell behaviour and, thereby, on pattern formation This work was funded by the Max-Planck-Gesellschaft |
| Databáze: | OpenAIRE |
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