Multiploid CD61+ cells are the pre-dominant cell lineage infected during acute dengue virus infection in bone marrow
| Autor: | Nattawat Onlamoon, Sansanee Noisakran, Kristina B. Clark, Francois Villinger, Guey Chuen Perng, John D. Roback, Aftab A. Ansari, Hui-Mien Hsiao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Viral Diseases
viruses lcsh:Medicine Dengue virus medicine.disease_cause Dengue fever Dengue 0302 clinical medicine Bone Marrow Molecular Cell Biology Platelet lcsh:Science Infectivity 0303 health sciences Multidisciplinary Integrin beta3 Hematology 3. Good health Infectious Diseases medicine.anatomical_structure P-Aminoazobenzene p-Aminoazobenzene Medicine Cellular Types Megakaryocytes Research Article Platelets Cell type Bone Marrow Cells Biology Microbiology Virus Colony-Forming Units Assay 03 medical and health sciences Virology medicine Animals Humans Cell Lineage 030304 developmental biology lcsh:R Dengue Virus medicine.disease Macaca mulatta Thrombocytopenia Immunology lcsh:Q Bone marrow Viral Transmission and Infection 030215 immunology |
| Zdroj: | PLoS ONE, Vol 7, Iss 12, p e52902 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Depression of the peripheral blood platelet count during acute infection is a hallmark of dengue. This thrombocytopenia has been attributed, in part, to an insufficient level of platelet production by megakaryocytes that reside in the bone marrow (BM). Interestingly, it was observed that dengue patients experience BM suppression at the onset of fever. However, few studies focus on the interaction between dengue virus (DENV) and megakaryocytes and how this interaction can lead to a reduction in platelets. In the studies reported herein, BM cells from normal healthy rhesus monkeys (RM) and humans were utilized to identify the cell lineage(s) that were capable of supporting virus infection and replication. A number of techniques were employed in efforts to address this issue. These included the use of viral RNA quantification, nonstructural protein and infectivity assays, phenotypic studies utilizing immunohistochemical staining, anti-differentiation DEAB treatment, and electron microscopy. Cumulative results from these studies revealed that cells in the BM were indeed highly permissive for DENV infection, with human BM having higher levels of viral production compared to RM. DENV-like particles were predominantly observed in multi-nucleated cells that expressed CD61+. These data suggest that megakaryocytes are likely the predominant cell type infected by DENV in BM, which provides one explanation for the thrombocytopenia and the dysfunctional platelets characteristic of dengue virus infection. |
| Databáze: | OpenAIRE |
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