In Vitro Induction of Neospora caninum Bradyzoites in Vero Cells Reveals Differential Antigen Expression, Localization, and Host-Cell Recognition of Tachyzoites and Bradyzoites
| Autor: | Camilla Björkman, Arunasalam Naguleswaran, Gereon Schares, Nathalie Vonlaufen, Norbert Müller, Daniela von Blumroeder, Nicole Guetg, Andrew Hemphill, John Ellis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Nitroprusside
Immunology Fluorescent Antibody Technique Antigens Protozoan Vacuole Biology Microbiology Cercopithecus aethiops Host-Parasite Interactions Neospora Antigen Chlorocebus aethiops Cell Adhesion Animals Vero Cells Life Cycle Stages Intracellular parasite biology.organism_classification Neospora caninum In vitro Microscopy Electron Infectious Diseases Vero cell Electrophoresis Polyacrylamide Gel Parasitology Fungal and Parasitic Infections Dense granule |
| Zdroj: | Infection and Immunity. 72:576-583 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.72.1.576-583.2004 |
| Popis: | We report on an optimized method for the in vitro culture of tissue cyst-forming Neospora caninum bradyzoites in Vero cells and the separation of viable parasites from host cells. Treatment of tachyzoite-infected Vero cell cultures with 17 μM sodium nitroprusside for 8 days severely scaled down parasite proliferation, led to reduced expression of tachyzoite surface antigens, and induced the expression of the bradyzoite marker NcBAG1 and the cyst wall antigen recognized by the monoclonal antibody MAbCC2. Transmission electron microscopy demonstrated that intracellular parasites were located within parasitophorous vacuoles that were surrounded by a cyst wall-like structure, and the dense granule antigens NcGRA1, NcGRA2, and NcGRA7 were incorporated into the cyst wall. Adhesion-invasion assays employing purified tachyzoites and bradyzoites showed that tachyzoites adhered to, and invaded, Vero cells with higher efficiency than bradyzoites. However, removal of terminal sialic acid residues from either the host cell or the parasite surface increased the invasion of Vero cells by bradyzoites, but not tachyzoites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|