Rodlike Particles of Polydopamine-CdTe Quantum Dots: An Actuator As a Photothermal Agent and Reactive Oxygen Species-Generating Nanoplatform for Cancer Therapy
| Autor: | Domingo F. Barber, Enrique Cedeño, Ernesto Marín, Yadileiny Portilla, Greter A. Ortega, S. Del Sol-Fernández, Seshasai Srinivasan, Amin Reza Rajabzadeh, Edilso Reguera |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Universidad Autónoma de Madrid, Consejo Nacional de Ciencia y Tecnología (México), Natural Sciences and Engineering Research Council of Canada |
| Rok vydání: | 2021 |
| Předmět: |
Absorption (pharmacology)
Materials science Indoles Light Cell Survival Polymers media_common.quotation_subject Kinetics education Antineoplastic Agents Apoptosis 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Cell Line Tumor Quantum Dots medicine Cadmium Compounds Humans General Materials Science Internalization media_common chemistry.chemical_classification Reactive oxygen species Photosensitizing Agents Photothermal therapy 021001 nanoscience & nanotechnology Subcellular localization 0104 chemical sciences 3. Good health Oxidative Stress chemistry Quantum dot Biophysics Tellurium 0210 nano-technology Reactive Oxygen Species Oxidative stress |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1944-8252 |
| Popis: | Herein, novel rodlike CdTe@MPA-PDA particles based on polydopamine (PDA) loaded with CdTe quantum dots (QDs) capped with mercaptopropionic acid (CdTe@MPA QDs) with atypical chemical features are evaluated as a potential actuator for photothermal therapy and oxidative stress induction. Under mild conditions established for the safe and efficient use of lasers, temperature increases of 10.2 and 7.8 °C, photothermal conversion efficiencies of 37.7 and 26.2%, and specific absorption rates of 99 and 69 W/g were obtained for CdTe@MPA-PDA and traditional PDA particles in water, respectively. The particles were set to interact with the human breast adenocarcinoma cell line MDA-MB-231. A significant cellular uptake with the majority of particles colocalized into the lysosomes was obtained at a concentration of 100 μg/mL after 24 h. Additionally, CdTe@MPA-PDA and CdTe@MPA QDs showed significantly different internalization levels and loading kinetics profiles. For the first time, the thermal lens technique was used to demonstrate the stability of particle-like CdTe@MPA-PDA after heating at pH 7 and their migration within the heating region due to the thermodiffusion effect. However, under acidic pH-type lysosomes, a performance decrease in heating was observed, and the chemical feature of the particles was damaged as well. Besides, the internalized rodlike CdTe@MPA-PDA notably enhanced the induction of oxidative stress compared with PDA alone and CdTe@MPA QDs in MDA-MB-231 cells initiating apoptosis. Combining these effects suggests that after meticulous optimizations of the conditions, the CdTe@MPA-PDA particles could be used as a photothermal agent under mild conditions and short incubation time, allowing cytoplasmatic subcellular localization. On the other hand, the same particles act as cell killers by triggering reactive oxygen species after a longer incubation time and lysosomal subcellular localization due to the pH effect on the chemical morphology features of the CdTe@MPA-PDA particles. This work was supported in part by (i) the Spanish Ministerio de Ciencia, Innovación y Universidades, under the following projects: SAF2017-82223-R; D.F.B. and Y.P. (FPU15/1414 06170, Molecular Biosciences Program of the Universidad Autónoma de Madrid) received a predoctoral FPU grant from the same Ministry; (ii) Research Grants SIP-IPN 20195617 and CONACYT, Mexico 2016-01-2482; E.C. received a postdoctoral grant from the latter; and (iii) Natural Sciences and Engineering Research Council of Canada. |
| Databáze: | OpenAIRE |
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