Nanomagnetic System for Rapid Diagnosis of Acute Infection
| Autor: | Firdausi Qadri, Richelle C. Charles, Ralph Weissleder, Soo Jin Kim, Sohyeon Park, Hakho Lee, Jun S. Song, Farhana Khanam, Changwook Min, Taufiqur Rahman Bhuiyan, Kyungheon Lee, H. Y. Kim, Ki Soo Park, Rasheduzzaman Rashu, Edward T. Ryan, Jinwoo Cheon |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Iron Cell General Physics and Astronomy Acute infection Biosensing Techniques Cell Separation Proof of Concept Study Article Hemolysin Proteins 03 medical and health sciences Antigen Lab-On-A-Chip Devices medicine Humans General Materials Science Typhoid Fever Antibody-Producing Cells Magnetite Nanoparticles Antigens Bacterial Hybridomas biology General Engineering Oxides Bacterial Infections Salmonella typhi equipment and supplies Antibodies Bacterial Molecular biology eye diseases Peripheral blood Zinc 030104 developmental biology medicine.anatomical_structure Salmonella paratyphi A Potential biomarkers Acute Disease Immunology biology.protein Magnetic nanoparticles Antibody human activities Biosensor |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.7b06074 |
| Popis: | Pathogen activated antibody-secreting cells (ASCs) produce and secrete antigen-specific antibodies. ASCs are detectable in the peripheral blood as early as 3 days after antigen exposure, which makes ASCs a potential biomarker for early disease detection. Here we present a Magnetic Capture and Detection (MCD) assay for sensitive, on-site detection of ASCs. In this approach, ASCs are enriched through magnetic capture, and secreted antibodies are magnetically detected by a miniaturized nuclear magnetic resonance (μNMR) system. This approach is based entirely on magnetics which supports high contrast against biological background, and simplifies assay procedures. We advanced the MCD system by i) synthesizing magnetic nanoparticles (MNPs) with high magnetic moments for both cell capture and antibody detection, ii) developing a miniaturized magnetic device for high-yield cell capture, and iii) optimizing the μNMR assay for antibody detection. Antibody responses targeting hemolysin E (HlyE) can accurately identify individuals with acute enteric fever. As a proof-of-concept, we applied MCD to detect antibodies produced by HlyE-specific hybridoma cells. The MCD achieved high sensitivity in detecting antibodies secreted from as few as 5 hybridoma cells (50 cells/mL). Importantly, the assay could be performed with whole blood with minimal sample processing. |
| Databáze: | OpenAIRE |
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