High-throughput metagenome analysis of the Sarcoptes scabiei internal microbiota and in-situ identification of intestinal Streptomyces sp
| Autor: | Rebecca Waddell, Pearl M. Swe, Martha Zakrzewski, Katja Fischer, Kadaba S. Sriprakash |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Sodium Hypochlorite Firmicutes lcsh:Medicine Sarcoptes scabiei Streptomyces Article Actinobacteria Microbiology Feces 03 medical and health sciences 0302 clinical medicine Mite Animals lcsh:Science In Situ Hybridization Fluorescence Multidisciplinary biology Microbiota lcsh:R High-Throughput Nucleotide Sequencing Bacteroidetes biology.organism_classification Gastrointestinal Microbiome Parasite biology 030104 developmental biology Metagenome bacteria Female lcsh:Q Metagenomics Proteobacteria 030217 neurology & neurosurgery Symbiotic bacteria |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-47892-0 |
| Popis: | Multiple parasitic arthropods of medical importance depend on symbiotic bacteria. While the link between scabies and secondary bacterial infections causing post infective complications of Group A streptococcal and staphylococcal pyoderma is increasingly recognized, very little is known about the microbiota of Sarcoptes scabiei. Here we analyze adult female mite and egg metagenome datasets. The majority of adult mite bacterial reads matched with Enterobacteriaceae (phylum Proteobacteria), followed by Corynebacteriaceae (phylum Actinobacteria). Klebsiella was the most dominant genus (78%) and Corynebacterium constituted 9% of the assigned sequences. Scabies mite eggs had a more diverse microbial composition with sequences from Proteobacteria being the most dominant (75%), while Actinobacteria, Bacteroidetes and Firmicutes accounted for 23% of the egg microbiome sequences. DNA sequences of a potential endosymbiont, namely Streptomyces, were identified in the metagenome sequence data of both life stages. The presence of Streptomyces was confirmed by conventional PCR. Digital droplet PCR indicated higher Streptomyces numbers in adult mites compared to eggs. Streptomyces were localized histologically in the scabies mite gut and faecal pellets by Fluorescent In Situ Hybridization (FISH). Streptomyces may have essential symbiotic roles in the scabies parasite intestinal system requiring further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|