Identification and Functional Characterization of Sesquiterpene Synthases fromXanthium strumarium
| Autor: | Zhen-Qiu Li, Fangfang Chen, Changfu Li, Yansheng Zhang, Yuanjun Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Saccharomyces cerevisiae Plant Science Biology Sesquiterpene Sesquiterpene lactone 01 natural sciences Xanthium Gas Chromatography-Mass Spectrometry Transcriptome Lactones 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Sequence Analysis Protein Complementary DNA Botany Plant Oils RNA Messenger Phylogeny Plant Proteins chemistry.chemical_classification Alkyl and Aryl Transferases food and beverages Cell Biology General Medicine biology.organism_classification Yeast Biosynthetic Pathways 030104 developmental biology Biochemistry chemistry Germacrene Mutation Ectopic expression Sesquiterpenes 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology. 57:630-641 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcw019 |
| Popis: | Xanthium strumarium synthesizes various pharmacologically active sesquiterpenes. The molecular characterization of sesquiterpene biosynthesis in X. strumarium has not been reported so far. In this study, the cDNAs coding for three sesquiterpene synthases (designated as XsTPS1, XsTPS2 and XsTPS3) were isolated using the X. strumarium transcriptome that we recently constructed. XsTPS1, XsTPS2 and XsTPS3 were revealed to have primary activities forming germacrene D, guaia-4,6-diene and germacrene A, respectively, by either ectopic expression in yeast cells or purified recombinant protein-based in vitro assays. Quantitative real-time PCRs and metabolite analysis for the different plant parts showed that the transcript abundance of XsTPS1-XsTPS3 is consistent with the accumulation pattern of their enzymatic products, supporting their biochemical functions in vivo. In particular, we discovered that none of the XsTPS2 product, guaia-4,6-diene, can be detected in one of the X. strumarium cultivars used in this study (it was named the Hubei-cultivar), in which a natural deletion of two A bases in the XsTPS2 cDNA disrupts its activity, which further confirmed the proposed biochemical role of XsTPS2 in X. strumarium in vivo. |
| Databáze: | OpenAIRE |
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