In vitro exploration of the synergistic effect of alternating magnetic field mediated thermo-chemotherapy with doxorubicin loaded dual pH- and thermo-responsive magnetic nanocomposite carriers

Autor: Lilin Wang, Paul Southern, Olivier Sandre, Aziliz Hervault, Nguyen T. K. Thanh, Franck Couillaud
Přispěvatelé: UCL Healthcare Biomagnetic and Nanomaterials Laboratories, University College of London [London] (UCL), Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Imagerie moléculaire et thérapies innovantes en oncologie (IMOTION), Université de Bordeaux (UB), EPSRC (EP/M015157/1 and EP/M018016/1), AOARD (FA2386-17-1-4042 award), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), European Project: COST Action TD1402,RADIOMAG, Université de Bordeaux (UB)-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Team 3 LCPO : Polymer Self-Assembly & Life Sciences, Université de Bordeaux (UB)-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), BoRdeaux Institute in onCology (Inserm U1312 - BRIC), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
human glioblastoma cells (U-87)
Biomedical Engineering
pH-sensitive polymers
[SDV.CAN]Life Sciences [q-bio]/Cancer
02 engineering and technology
Magnetic nanoparticles MNPs
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
03 medical and health sciences
0302 clinical medicine
pH-responsive polymers
medicine
Bioluminescence imaging
General Materials Science
MTT assay
Doxorubicin
Magnetic hyperthermia
Cytotoxicity
human breast carcinoma cells (MCF-7)
half maximal inhibitory concentration (IC50)
Doxorubicin DOX
Chemistry
General Chemistry
General Medicine
021001 nanoscience & nanotechnology
3. Good health
[CHIM.POLY]Chemical Sciences/Polymers
030220 oncology & carcinogenesis
Valeriote’s statistical assay
Drug delivery
Biophysics
Nanocarriers
0210 nano-technology
synergistic efficiency
[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
medicine.drug
Zdroj: Journal of materials chemistry‎ B
Journal of materials chemistry‎ B, Royal Society of Chemistry, 2020, 8 (46), pp.10527-10539. ⟨10.1039/D0TB01983F⟩
Journal of materials chemistry‎ B, 2020, 8 (46), pp.10527-10539. ⟨10.1039/D0TB01983F⟩
ISSN: 2050-750X
DOI: 10.1039/D0TB01983F⟩
Popis: International audience; Nanoparticle induced hyperthermia has been considered as a promising approach for cancer treatment for decades. The local heating ability and drug delivery potential highlight a diversified possibility in clinical application, therefore a variety of nanoparticles that has been developed accordingly. However, currently, only a few of them have been translated into clinical stage indicating the ‘nanoparticle medically underserved’ situation, which encourages their comprehensive biomedical exploration. This study presents a thorough biological evaluation of previous well-developed dual pH- and thermo- responsive magnetic Doxorubicin-nanocarrier (MNC-DOX) in multiple cancer cell lines. The biocompatible of the nanocomposites has been proved by the MTT assay on primary cell lines. The histology and fluorescence microscopy imaging revealed the efficient but various cellular uptaking of nanocarriers among cell lines. The IC50 of MNC-DOX is significantly lower than free DOX without alternative magnetic field (AMF), which implied the potential to lower the systemic cytotoxicity in clinical research. The concurrent thermo-chemotherapy generated by this platform has been successfully achieved under alternating magnetic field (AMF). Promising effective synergistic results have been demonstrated through in vitro study in multi-model cancer cell lines via both trypan blue exclusion method and bioluminescence imaging method. Furthermore, the two most used magnetic hyperthermia modality, namely intracellular and extracellular treatments have been compared on the same nanocarriers in all 3 cell lines, which showed treatment after internalization is not required but preferable. These results lead to the conclusion that this dual responsive nanocarrier has extraordinary potential to serve as a novel broad-spectrum anticancer drug and worth to be pursued for potential clinical applications.
Databáze: OpenAIRE
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