Myosin VI in skeletal muscle: its localization in the sarcoplasmic reticulum, neuromuscular junction and muscle nuclei
| Autor: | Magdalena Sobczak, Grzegorz M. Wilczynski, Urszula Sławińska, Maria Jolanta Redowicz, Marine Yeghiazaryan, Elisabeth Ehler, Anna Jakubiec-Puka, Łukasz Majewski, Justyna Karolczak |
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| Rok vydání: | 2012 |
| Předmět: |
Myofilament
medicine.medical_specialty Histology Muscle Fibers Skeletal Neuromuscular Junction Synaptic Membranes Sarcoplasmic reticulum Biology Sarcomere Nucleus Mice Internal medicine Myosin medicine Myocyte Animals Myocytes Cardiac Rats Wistar Molecular Biology Actin Cytoskeleton Muscle fiber Cell Nucleus Original Paper Myosin Heavy Chains Cardiac muscle Skeletal muscle Cell Biology Actin cytoskeleton Denervation Cell biology Hindlimb Rats Mice Inbred C57BL Medical Laboratory Technology medicine.anatomical_structure Endocrinology Myosin VI Female Protein Binding |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X |
| Popis: | Myosin VI (MVI) is a unique unconventional motor moving backwards on actin filaments. In non-muscle cells, it is involved in cell migration, endocytosis and intracellular trafficking, actin cytoskeleton dynamics, and possibly in gene transcription. An important role for MVI in striated muscle functioning was suggested in a report showing that a point mutation (H236R) within the MVI gene was associated with cardiomyopathy (Mohiddin et al., J Med Genet 41:309–314, 2004). Here, we have addressed MVI function in striated muscle by examining its expression and distribution in rat hindlimb skeletal muscle. We found that MVI was present predominantly at the muscle fiber periphery, and it was also localized within muscle nuclei. Analysis of both the hindlimb and cardiac muscle longitudinal sections revealed ~3 μm striation pattern, corresponding to the sarcoplasmic reticulum. Moreover, MVI was detected in the sarcoplasmic reticulum fractions isolated from skeletal and cardiac muscle. The protein also localized to the postsynaptic region of the neuromuscular junction. In denervated muscle, the defined MVI distribution pattern was abolished and accompanied by significant increase in its amount in the muscle fibers. In addition, we have identified several novel potential MVI-binding partners, which seem to aid our observations that in striated muscle MVI could be involved in postsynaptic trafficking as well as in maintenance of and/or transport within the sarcoplasmic reticulum and non-sarcomeric cytoskeleton. Electronic supplementary material The online version of this article (doi:10.1007/s00418-012-1070-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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