Nanofibrillated cellulose-enzyme assemblies for enhanced biotransformations with in situ cofactor regeneration
| Autor: | Natalia Calixto Mancipe, Han Seung Yang, William Tai Yin Tze, Benjiamin Frigo-Vaz, Ping Wang, Marcia Cristina Branciforti, Guoying Dai |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Nanofibers Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Cofactor Catalysis Nanocellulose Clostridium thermocellum Industrial Microbiology chemistry.chemical_compound Protein Domains Biotransformation Multienzyme Complexes 010608 biotechnology NADH NADPH Oxidoreductases Bioprocess Cellulose Molecular Biology chemistry.chemical_classification MATERIAIS L-Lactate Dehydrogenase biology 010405 organic chemistry Temperature General Medicine Cellulose binding Combinatorial chemistry Recombinant Proteins Nanostructures 0104 chemical sciences Lacticaseibacillus casei Enzyme chemistry biology.protein Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | We report herein the use of nanofibrillated cellulose (NFC) for development of enzyme assemblies in an oriented manner for biotransformation with in situ cofactor regeneration. This is achieved by developing fusion protein enzymes with cellulose-specific binding domains. Specifically, lactate dehydrogenase and NADH oxidase were fused with a cellulose binding domain, which enabled both enzyme recovery and assembling in essentially one single step by using NFC. Results showed that the binding capacity of the enzymes was as high as 0.9 μmol-enzyme/g-NFC. Compared to native parent free enzymes, NFC-enzyme assemblies improved the catalytic efficiency of the coupled reaction system by over 100%. The lifetime of enzymes was also improved by as high as 27 folds. The work demonstrates promising potential of using biocompatible and environmentally benign bio-based nanomaterials for construction of efficient catalysts for intensified bioprocessing and biotransformation applications. |
| Databáze: | OpenAIRE |
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