Pigment production by Fusarium solani BRM054066 and determination of antioxidant and anti-inflammatory properties
| Autor: | Aparecido Almeida Conceição, Manoel Neres Santos Junior, Kirley Marques Canuto, Simone Mendonça, Bruna dos Santos Menezes, Lucas Miranda Marques, Lucas Santos Solidade, Patrícia Lopes Leal, Félix Gonçalves de Siqueira, Edy Sousa de Brito |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
DPPH medicine.drug_class medicine.medical_treatment lcsh:Biotechnology Biophysics lcsh:QR1-502 01 natural sciences Applied Microbiology and Biotechnology Anthraquinone Anti-inflammatory lcsh:Microbiology Bioactive compounds 03 medical and health sciences Pigment chemistry.chemical_compound lcsh:TP248.13-248.65 medicine Food science 030304 developmental biology 0303 health sciences biology 010405 organic chemistry Biological activity Nuclear magnetic resonance spectroscopy Doehlert design biology.organism_classification 0104 chemical sciences Submerged fermentation chemistry visual_art visual_art.visual_art_medium Original Article Natural red pigment Fusarium solani |
| Zdroj: | AMB Express AMB Express, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2191-0855 |
| Popis: | The fungal kingdom has been widely studied as a source of bioactive compounds of interest to the pharmaceutical and food industry. This paper studies the production of natural red pigments by Fusarium solani BRM054066 in the submerged fermentation system, using Doehlert experimental design to determine optimal cultivation conditions. The chemical composition of the red pigment was determined by Nuclear Magnetic Resonance spectroscopy (NMR) and Ultra-Performance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MS). Antioxidant activity was assessed by the ability to sequester of free radical DPPH. In the analysis of anti-inflammatory activity, murine peritoneal macrophages activated by LPS were used, and the gene expression of TNF-α, IL-1β, IL-6, IL-10 and IL-17 was determined using qPCR. As a result, it was found that agitation at 200 rpm and glucose concentration ≥ 20 g/L promote the best results in the production of red pigment. The chemical compounds identified were two naphthoquinones, fusarubin and dihydrofusarubin, and an anthraquinone, a bostrycoidin, being fusarubin the majority compound. The red pigment showed antioxidant activity by scavenge 50% of the DPPH radical, in a concentration of 24 µg/mL. The pigment also showed an effective anti-inflammatory capacity by reducing the overexpression of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 and promoting the production of anti-inflammatory IL-10 and IL-17, in murine macrophages activated by LPS (p F. solani BRM054066, under optimized conditions of cultivation, proved to be a promising source of biologically active natural pigments with wide industrial applicability. |
| Databáze: | OpenAIRE |
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