The Effect of α-Interferon on Thymidine, Uridine, and Leucine Uptake and Ultrastructure of Peripheral Blood Mononuclear Cells from Multiple Myeloma Patients
| Autor: | M. Djaldetti, A. Winder, Amos Cohen, P. Fishman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism Lymphocyte medicine.medical_treatment Alpha interferon Interferon alpha-2 Biology Biochemistry Peripheral blood mononuclear cell chemistry.chemical_compound Leucine medicine Humans Lymphocytes Uridine Interferon alfa Aged Interferon-alpha Molecular biology Recombinant Proteins medicine.anatomical_structure Cytokine chemistry Leukocytes Mononuclear Microscopy Electron Scanning Female Multiple Myeloma Thymidine medicine.drug |
| Zdroj: | Biochemical Medicine and Metabolic Biology. 49:124-132 |
| ISSN: | 0885-4505 |
| DOI: | 10.1006/bmmb.1993.1015 |
| Popis: | The effect of IFN α-2b on thymidine, uridine, and leucine uptake was examined on peripheral blood mononuclear cells (PBMC) of healthy donors and 15 patients with multiple myeloma (MM). In addition, the surface ultrastructure of the cells incubated without or with IFN α-2b was examined with a scanning electron microscope. The results showed that IFN had no effect on thymidine, uridine, or leucine uptake of unstimulated MM and control PBMC. On the other hand IFN inhibited thymidine, and uridine uptake of PWM-stimulated MM PBMC, but had no effect on healthy donor stimulated PBMC. 1FN inhibited also thymidine and uridine uptake in PHA-stimulated healthy donors and MM patients′ PBMC. The cellular surface ultrastructure of MM lymphocytes incubated with 100 u/ml IFN showed disappearance of the microvilli and formation of cellular pits, whereas in healthy donor lymphocytes IFN caused flattening of microvilli. |
| Databáze: | OpenAIRE |
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