Microspheres Containing Cibacron Blue F3G-A and Incorporated Iron Oxide Nanoparticles as Biomarker Harvesting Platforms
| Autor: | Alexis Patanarut, Barney Bishop, Lance A. Liotta, Emanuel F. Petricoin, Elissa H. Williams |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
In situ
Cibacron Blue F3G-A Materials science Polymers and Plastics Coprecipitation 02 engineering and technology 010402 general chemistry 01 natural sciences lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry hydrogels Chromatography Cibacron blue iron oxide nanoparticles General Chemistry 021001 nanoscience & nanotechnology equipment and supplies 0104 chemical sciences Chemical engineering chemistry Acrylamide Self-healing hydrogels Particle Magnetic nanoparticles 0210 nano-technology human activities Iron oxide nanoparticles affinity dyes |
| Zdroj: | Polymers, Vol 3, Iss 3, Pp 1181-1198 (2011) Polymers Volume 3 Issue 3 Pages 1181-1198 |
| ISSN: | 2073-4360 |
| Popis: | In this work, magnetic functionality was introduced to cross-linked acrylamide-based particles via the in situ coprecipitation of iron oxide nanoparticles within the hydrogel particle interior. Cibacron Blue F3G-A was then incorporated onto the magnetic hydrogel scaffold to facilitate the harvest of targeted protein species. The dye-loaded magnetic particles were physically characterized, and their protein sequestration performance was investigated. The results of these studies indicated that dye-loaded magnetic particles sequestered a greater amount of lower molecular weight proteins from the test solution than was achieved using reference particles, dye-loaded cross-linked N-isopropylacrylamide-based core-shell particles. This difference in protein harvesting ability may reflect the higher degree of dye-loading in the magnetic particles relative to the dye-loaded core-shell particles. |
| Databáze: | OpenAIRE |
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