Corticotropin-releasing factor and urocortin differentially modulate rat Purkinje cell dendritic outgrowth and differentiation in vitro

Autor: Albert Gramsbergen, Natalia V. Gounko, J. J. L. van der Want, J. IJkema-Paassen, Friedrich Metzger, Jerome D. Swinny
Rok vydání: 2004
Předmět:
Calbindins
Cerebellum
Indoles
Time Factors
Corticotropin-Releasing Hormone
Cellular differentiation
Purkinje cell
Cell Count
Hippocampus
DESENSITIZATION
stress
Purkinje Cells
POTENTIAL MECHANISM
PKA
Drug Interactions
Enzyme Inhibitors
Urocortins
Urocortin
trophic
General Neuroscience
LOCALIZATION
Cell Differentiation
FACTOR RECEPTORS
Immunohistochemistry
Neuroprotective Agents
medicine.anatomical_structure
Signal transduction
hormones
hormone substitutes
and hormone antagonists
endocrine system
medicine.medical_specialty
cerebellum
Carbazoles
In Vitro Techniques
Biology
SIGNALING PATHWAYS
S100 Calcium Binding Protein G
Internal medicine
medicine
Animals
Humans
Pyrroles
PROTEIN-KINASE-C
Protein kinase A
Protein kinase C
MOUSE CEREBELLUM
Flavonoids
Analysis of Variance
Dose-Response Relationship
Drug
HIPPOCAMPAL-NEURONS
CENTRAL-NERVOUS-SYSTEM
Dendrites
MAPK
Rats
Pyrimidines
Endocrinology
Animals
Newborn
Linear Models
CEREBELLAR SLICE CULTURES
Zdroj: European Journal of Neuroscience, 19(7), 1749-1758. Blackwell Publishing
ISSN: 1460-9568
0953-816X
DOI: 10.1111/j.1460-9568.2004.03279.x
Popis: The precise outgrowth and arborization of dendrites is crucial for their function as integrators of signals relayed from axons and, hence, the functioning of the brain. Proper dendritic differentiation is particularly resonant for Purkinje cells as the intrinsic activity of this cell-type is governed by functionally distinct regions of its dendritic tree. Activity-dependent mechanisms, driven by electrical signaling and trophic factors, account for the most active period of dendritogenesis. An as yet unexplored trophic modulator of Purkinje cell dendritic development is corticotropin-releasing factor (CRF) and family member, urocortin, both of which are localized in climbing fibers. Here, we use rat organotypic cerebellar slice cultures to investigate the roles of CRF and urocortin on Purkinje cell dendritic development. Intermittent exposure (12 h per day for 10 days in vitro) of CRF and urocortin induced significantly more dendritic outgrowth (45% and 70%, respectively) and elongation (25% and 15%, respectively) compared with untreated cells. Conversely, constant exposure to CRF and urocortin significantly inhibited dendritic outgrowth. The trophic effects of CRF and urocortin are mediated by the protein kinase A and mitogen-activating protein kinase pathways. The study shows unequivocally that CRF and urocortin are potent regulators of dendritic development. However, their stimulatory or inhibitory effects are dependent upon the degree of expression of these peptides. Furthermore, the effects of CRF and urocortin on neuronal differentiation and re-modeling may provide a cellular basis for pathologies such as major depression, which show perturbations in the expression of these stress peptides.
Databáze: OpenAIRE
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