Phenol Extraction Op 28 S Ribosomal RNA Prom Rat Liver Cytoplasm

Autor:	M. D. Dabeva
Rok vydání:	1976
Předmět:	Electrophoresis Agar Gel Gel electrophoresis Cytoplasm Density gradient Phenol extraction Ribosomal RNA Biology Biochemistry Rats chemistry.chemical_compound Liver Phenols chemistry RNA Ribosomal Rat liver Methods Genetics Animals Phenol Interphase
Zdroj:	Preparative Biochemistry. 6:285-294
ISSN:	0032-7484
Popis:	A simple and reproducible phenol method for the isolation of 28 S ribosomal RNA from rat liver cytoplasm, free from poly(A)-RNA is described. The procedure is based on the observation that at lower pH of the homogenate (pH 5.5) 28 S ribosomal RNA is extracted, while 18 S ribosomal RNA remains in the interphase layer. Isolation of pure 28 S or 18 S ribosomal RNA in preparative amounts requires density gradient cen-trifugation or preparative gel electrophoresis. In this communication a rapid and reproducible method for the isolation of 28 S ribosomal RNA is proposed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b56d842259a723e5e2cd71266269cc5e https://doi.org/10.1080/00327487608061619 Zobrazit plný text záznamu