An RNA thermoswitch regulates daytime growth in Arabidopsis
| Autor: | Martin Balcerowicz, Ian Brierley, Krzysztof Franaszek, Andrew E. Firth, Philip A. Wigge, Poppy Marriott, Betty Y.-W. Chung, Feng Geng, Marco Di Antonio, Katja E. Jaeger |
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| Přispěvatelé: | Chung, Betty YW [0000-0003-4384-285X], Balcerowicz, Martin [0000-0001-9462-4436], Di Antonio, Marco [0000-0002-7321-1867], Wigge, Philip A [0000-0003-4822-361X], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Plant Science 01 natural sciences Article 03 medical and health sciences Gene Expression Regulation Plant Transcription (biology) Gene expression Transcription factor Gene Regulation of gene expression Messenger RNA biology Arabidopsis Proteins Chemistry Temperature RNA biology.organism_classification Circadian Rhythm Cell biology DNA-Binding Proteins 030104 developmental biology RNA Plant Transcription Factors 010606 plant biology & botany |
| Zdroj: | Nat Plants |
| ISSN: | 2055-0278 |
| DOI: | 10.1038/s41477-020-0633-3 |
| Popis: | Temperature is a major environmental cue affecting plant growth and development. Plants often experience higher temperatures in the context of a 24 h day–night cycle, with temperatures peaking in the middle of the day. Here, we find that the transcript encoding the bHLH transcription factor PIF7 undergoes a direct increase in translation in response to warmer temperature. Diurnal expression of PIF7 transcript gates this response, allowing PIF7 protein to quickly accumulate in response to warm daytime temperature. Enhanced PIF7 protein levels directly activate the thermomorphogenesis pathway by inducing the transcription of key genes such as the auxin biosynthetic gene YUCCA8, and are necessary for thermomorphogenesis to occur under warm cycling daytime temperatures. The temperature-dependent translational enhancement of PIF7 messenger RNA is mediated by the formation of an RNA hairpin within its 5′ untranslated region, which adopts an alternative conformation at higher temperature, leading to increased protein synthesis. We identified similar hairpin sequences that control translation in additional transcripts including WRKY22 and the key heat shock regulator HSFA2, suggesting that this is a conserved mechanism enabling plants to respond and adapt rapidly to high temperatures. Temperature-dependent translational enhancement of PIF7 promotes gene expression for thermomorphogenesis in Arabidopsis during warm daytime. This enhanced translation is mediated by an RNA hairpin which shifts conformation at higher temperatures. |
| Databáze: | OpenAIRE |
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