Primary and Chronic HIV Infection Differently Modulates Mucosal Vδ1 and Vδ2 T-Cells Differentiation Profile and Effector Functions
| Autor: | Alessandra Sacchi, Federico Martini, Paola Scognamiglio, Raffaella Lionetti, Veronica Bordoni, Rita Casetti, Nicola Tumino, Eleonora Cimini, Chrysoula Vlassi, Chiara Agrati, Gianpiero D'Offizi |
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| Přispěvatelé: | Cimini, E., Agrati, C., D'Offizi, G., Vlassi, C., Casetti, R., Sacchi, A., Lionetti, R., Bordoni, V., Tumino, N., Scognamiglio, P., Martini, F. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Cellular differentiation lcsh:Medicine HIV Infections Stimulation Biology Lymphocyte Activation Flow cytometry Immune system T-Lymphocyte Subsets medicine Humans Cytotoxic T cell lcsh:Science Immunity Mucosal Multidisciplinary medicine.diagnostic_test Effector T-cell receptor lcsh:R virus diseases Cell Differentiation Receptors Antigen T-Cell gamma-delta Middle Aged Chronic infection Chronic Disease Immunology Female lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 6, p e0129771 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Gut-associated immune system has been identified as a major battlefield during the early phases of HIV infection. γδ T-cells, deeply affected in number and function after HIV infection, are able to act as a first line of defence against invading pathogens by producing antiviral soluble factors and by killing infected cells. Despite the relevant role in mucosal immunity, few data are available on gut-associated γδ T-cells during HIV infection. Aim of this work was to evaluate how primary (P-HIV) and chronic (C-HIV) HIV infection affects differentiation profile and functionality of circulating and gut-associated Vδ1 and Vδ2 T-cells. In particular, circulating and mucosal cells were isolated from respectively whole blood and residual gut samples from HIV-infected subjects with primary and chronic infection and from healthy donors (HD).Differentiation profile and functionality were analyzed by multiparametric flow cytometry. P-HIV and C-HIV were characterized by an increase in the frequency of effector Vδ1-T cells both in circulating and mucosal compartments. Moreover, during PHIV mucosal Vδ1 T-cells expressed high levels of CD107a, suggesting a good effector cytotoxic capability of these cells in the early phase of infection that was lost in C-HIV. P-HIV induced an increase in circulating effector Vδ2 T-cells in comparison to C-HIV and HD. Notably, P-HIV as well as HD were characterized by the ability of mucosal Vδ2 T-cells to spontaneously produce IFN-γ that was lost in C-HIV. Altogether, our data showed for the first time a functional capability of mucosal Vδ1 and Vδ2 T-cells during P-HIV that was lost in C-HIV, suggesting exhaustion mechanisms induced by persistent stimulation. Copyright |
| Databáze: | OpenAIRE |
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