New Sensitive Competitive Enzyme-Linked Immunosorbent Assay Using a Monoclonal Antibody against Nonstructural Protein 1 of West Nile Virus NY99
Autor: | Shinya Shimizu, Jiro Hirota, Yoshihiro Shimoji |
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Rok vydání: | 2012 |
Předmět: |
Microbiology (medical)
medicine.drug_class viruses Clinical Biochemistry Immunology Encephalitis Virus St. Louis Enzyme-Linked Immunosorbent Assay Cross Reactions Viral Nonstructural Proteins Biology Antibodies Viral Monoclonal antibody Virus Western blot Antibody Specificity Diagnostic Laboratory Immunology Veterinary virology medicine Animals Immunology and Allergy Encephalitis Virus Japanese chemistry.chemical_classification medicine.diagnostic_test Bird Diseases virus diseases Antibodies Monoclonal Japanese encephalitis medicine.disease Virology Enzyme chemistry biology.protein Antibody Chickens West Nile virus West Nile Fever Encephalitis |
Zdroj: | Clinical and Vaccine Immunology. 19:277-283 |
ISSN: | 1556-679X 1556-6811 |
Popis: | An anti-West Nile virus (anti-WNV) monoclonal antibody, SHW-7A11, was developed for competitive enzyme-linked immunosorbent assays (c-ELISAs). SHW-7A11 reacted with nonstructural protein 1 in Western blot analysis. SHW-7A11 was relatively specific for the WNV strain NY99 and recognized Kunjin and Eg101 strains in indirect ELISAs. Two c-ELISAs were developed for sera diluted 10 and 100 times and named c-ELISA10 and c-ELISA100, respectively. Both c-ELISAs detected antibodies against WNV NY99 and Kunjin strains. Little cross-reactivity was observed for antibodies against Japanese encephalitis virus and St. Louis encephalitis virus in these assays. Using the cutoff point for the St. Louis encephalitis virus, all WNV-infected chickens were found to be positive on day 21 after infection in both c-ELISAs. On the other hand, all infected chickens were found to be positive on day 35 after infection in a virus neutralization test. Our newly developed SHW-7A11-based c-ELISA can detect WNV infection with sera diluted 10 to 100 times. Therefore, this c-ELISA can be used for WNV serosurveillance of chickens and wild birds. |
Databáze: | OpenAIRE |
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