Isothiazolinone Detection in Dish Soap and Personal Care Products: Comparison of Lovibond Isothiazolinone Test Kit and Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry
| Autor: | Lun-Yi Zang, Erin M. Warshaw, Paul D. Siegel, Rebecca S. Kimyon, Lindsey M. Voller, Jamie P. Schlarbaum |
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| Rok vydání: | 2020 |
| Předmět: |
Cosmetics
Dermatology Soaps Article Isothiazolinone 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liquid chromatography–mass spectrometry Methylisothiazolinone medicine Immunology and Allergy 030212 general & internal medicine Allergic contact dermatitis Chromatography High Pressure Liquid Personal care Chromatography business.industry Methylchloroisothiazolinone medicine.disease Thiazoles chemistry Contact allergy CRITERION STANDARD business |
| Zdroj: | Dermatitis |
| ISSN: | 2162-5220 1710-3568 |
| Popis: | Background Isothiazolinones are commonly used preservatives, which may cause allergic contact dermatitis. The Lovibond Isothiazolinone Test Kit (LITK) has been reported to successfully identify clinically relevant, occult isothiazolinones in patient personal care products. Objective The aim of the study was to analyze dish soaps and personal care products that do not declare isothiazolinones ("no-ISO") for the presence of isothiazolinones via 2 methods: LITK and ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Methods No-ISO dish soaps (n = 9), a convenience sample of patient products (n = 6), and controls (positive [isothiazolinone declared], n = 5; negative, n = 2) were tested with LITK (X3) and UHPLC-MS/MS. Results Several no-ISO dish soaps and personal products were positive for isothiazolinones (LITK, n = 12; UHPLC-MS/MS, n = 3). Ultrahigh-performance liquid chromatography-tandem mass spectrometry specifically identified methylisothiazolinone alone in 1 no-ISO dish soap, methylchloroisothiazolinone in another, and both in a third. Using UHPLC-MS/MS as the criterion standard, we observed the accuracy of LITK for 9 dish soaps was poor (sensitivity, 66.7%; specificity, 20%) and very poor for 6 personal care products (sensitivity, 0%; specificity, 0%). Conclusions Personal products may contain undeclared isothiazolinones. The current study found that LITK had poor accuracy for testing dish soap and personal care products. Clinicians should be aware of these factors when managing patients with contact allergy to isothiazolinones. |
| Databáze: | OpenAIRE |
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