Innate and Adaptive Mediators in Cystic Fibrosis and Allergic Fungal Rhinosinusitis
| Autor: | Jeffrey W. Hall, Rodney J. Schlosser, Danforth A. Newton, Bradford A. Woodworth, Margaret Leigh Skinner, John E. Baatz, Jeffrey G. Neal, Deanne M. R. Lathers |
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| Rok vydání: | 2007 |
| Předmět: |
Chemokine CCL11
Rhinitis Allergic Perennial Cystic Fibrosis Inflammation Respiratory Mucosa Models Biological Cystic fibrosis Fungal Proteins 03 medical and health sciences 0302 clinical medicine Immune system Immunity medicine Humans 030223 otorhinolaryngology Sinus (anatomy) Mucous Membrane Innate immune system Pulmonary Surfactant-Associated Protein A Tumor Necrosis Factor-alpha business.industry Mucous membrane Th1 Cells Pulmonary Surfactant-Associated Protein D medicine.disease Acquired immune system Immunity Innate medicine.anatomical_structure Gene Expression Regulation Otorhinolaryngology Immune System 030220 oncology & carcinogenesis Immunology medicine.symptom business |
| Zdroj: | American Journal of Rhinology. 21:538-541 |
| ISSN: | 1539-6290 1050-6586 |
| DOI: | 10.2500/ajr.2007.21.3070 |
| Popis: | BackgroundSurfactant-associated proteins (SP) A and D are both innate immunity mediators and produced in normal and diseased sinus mucosa. Cystic fibrosis (CF) is associated with Th1 adaptive inflammation whereas allergic fungal rhinosinusitis (AFRS) is associated with Th2 adaptive inflammation. The purpose of this study is to show and quantify the presence of SP A, SP D, tumor necrosis factor (TNF) alpha, (a Th1 marker), and eotaxin (a Th2 marker) in normal and diseased sinus mucosa.MethodsIntraoperative sinus mucosal biopsy specimens from human volunteers were obtained during endoscopic sinus surgery for CF (n = 4), AFRS (n = 10), and normal controls (CTLs; n = 4). Specimens were evaluated for presence and quantity of SP A, SP D, and TNF-alpha using Western blot with semiquantitative immunoblot analysis. Eotaxin was quantified using ELISA immunoassay. Results were standardized and reported as picograms of mediator per microgram of total protein.ResultsSP A, SP D, and TNF-alpha levels in CF tissue extracts were 2–10 times higher than levels in AFRS tissue (with SP D and TNF-alpha reaching statistical significance) but CF tissue was not significantly higher than CTL tissue. SP A, SP D, and TNF-alpha were not significantly elevated in AFRS. Eotaxin showed elevated levels in CF and AFRS when compared with CTLs (p = 0.03 and 0.003, respectively).ConclusionSP D and TNF-alpha are significantly increased in CF compared with AFRS, suggesting activation of both innate immunity and Th1-mediated inflammation and potential correlation between SPs and downstream adaptive immune responses. |
| Databáze: | OpenAIRE |
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